Construct: ORF TRCN0000472969
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010131.1_s317c1
- Derived from:
- ccsbBroadEn_01338
- DNA Barcode:
- GTTCGGTCTCAGATTGTGCCTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTH2R (5746)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472969
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_005048.4 | 100% | 100% | |
| 2 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001309516.2 | 79.8% | 79.8% | 0_1ins333 |
| 3 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001371905.1 | 79.8% | 79.8% | 0_1ins333 |
| 4 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001371906.1 | 79.8% | 79.8% | 0_1ins333 |
| 5 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001371907.1 | 63% | 63% | 0_1ins333;647_648ins276 |
| 6 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163993.1 | 53.1% | (many diffs) | |
| 7 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163994.1 | 52.6% | (many diffs) | |
| 8 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163991.1 | 51.8% | (many diffs) | |
| 9 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163992.1 | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1716
- ORF length:
- 1650
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgggctgggg gcgtcgctcc acgtctgggg ttggctaatg ctcggcagct 121 gcctcctggc cagagcccag ctggattctg atggcaccat tactatagag gagcagattg 181 tccttgtgct gaaagcgaaa gtacaatgtg aactcaacat cacagctcaa ctccaggagg 241 gagaaggtaa ttgtttccct gaatgggatg gactcatttg ttggcccaga ggaacagtgg 301 ggaaaatatc ggctgttcca tgccctcctt atatttatga cttcaaccat aaaggagttg 361 ctttccgaca ctgtaacccc aatggaacat gggattttat gcacagctta aataaaacat 421 gggccaatta ttcagactgc cttcgctttc tgcagccaga tatcagcata ggaaagcaag 481 aattctttga acgcctctat gtaatgtata ccgttggcta ctccatctct tttggttcct 541 tggctgtggc tattctcatc attggttact tcagacgatt gcattgcact aggaactata 601 tccacatgca cttatttgtg tctttcatgc tgagagctac aagcatcttt gtcaaagaca 661 gagtagtcca tgctcacata ggagtaaagg agctggagtc cctaataatg caggatgacc 721 cacaaaattc cattgaggca acttctgtgg acaaatcaca atatatcggg tgcaagattg 781 ctgttgtgat gtttatttac ttcctggcta caaattatta ttggatcctg gtggaaggtc 841 tctacctgca taatctcatc tttgtggctt tcttttcgga caccaaatac ctgtggggct 901 tcatcttgat aggctggggg tttccagcag catttgttgc agcatgggct gtggcacgag 961 caactctggc tgatgcgagg tgctgggaac ttagtgctgg agacatcaag tggatttatc 1021 aagcaccgat cttagcagct attgggctga attttattct gtttctgaat acggttagag 1081 ttctagctac caaaatctgg gagaccaatg cagttgggca tgacacaagg aagcaataca 1141 ggaaactggc caaatcgaca ctggtcctgg tcctagtctt tggagtgcat tacatcgtgt 1201 tcgtatgcct gCCTCACTCC TTCACTGGGC TCGGGTGGGA GATCCGCATG CACTGTGAGC 1261 TCTTCTTCAA CTCCTTTCAG GGTTTCTTTG TGTCTATCAT CTACTGCTAC TGCAATGGAG 1321 AGGTTCAGGC AGAGGTGAAG AAGATGTGGA GTCGGTGGAA CCTCTCCGTG GACTGGAAAA 1381 GGACACCGCC ATGTGGCAGC CGCAGATGCG GCTCAGTGCT CACCACCGTG ACGCACAGCA 1441 CCAGCAGCCA GTCACAGGTG GCGGCCAGCA CACGCATGGT GCTTATCTCT GGCAAAGCTG 1501 CCAAGATCGC CAGCAGACAG CCTGACAGCC ACATCACTTT ACCTGGCTAT GTCTGGAGTA 1561 ACTCAGAGCA GGACTGCCTG CCACACTCTT TCCACGAGGA GACCAAGGAA GATAGTGGGA 1621 GGCAGGGAGA TGATATTCTA ATGGAGAAGC CTTCCAGGCC TATGGAATCT AACCCAGACA 1681 CTGAAGGATG CCAAGGAGAA ACTGAGGATG TTCTCTGCCC AACTTTCTTG TACAAAGTGG 1741 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1801 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1861 AGTTCGGTCT CAGATTGTGC CTACCACGCG TTAAGTCgac aatcaacctc tggattacaa 1921 aatttgtgaa agatt