Transcript: Human NM_001309516.2

Homo sapiens parathyroid hormone 2 receptor (PTH2R), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
PTH2R (5746)
Length:
2971
CDS:
805..2124

Additional Resources:

NCBI RefSeq record:
NM_001309516.2
NBCI Gene record:
PTH2R (5746)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001309516.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000358063 CTCATATATATCACCCTAAAT pLKO_005 2443 3UTR 100% 13.200 18.480 N PTH2R n/a
2 TRCN0000014195 GCACCGATCTTAGCAGCTATT pLKO.1 1429 CDS 100% 10.800 15.120 N PTH2R n/a
3 TRCN0000358061 CCATGCTCACATAGGAGTAAA pLKO_005 1074 CDS 100% 13.200 9.240 N PTH2R n/a
4 TRCN0000014193 GCCTGTGCATAGGAGCAATTA pLKO.1 2559 3UTR 100% 13.200 9.240 N PTH2R n/a
5 TRCN0000358062 TCTGATGGCACCATTACTATA pLKO_005 553 5UTR 100% 13.200 9.240 N PTH2R n/a
6 TRCN0000014194 CCCTCCTTATATTTATGACTT pLKO.1 729 5UTR 100% 4.950 3.465 N PTH2R n/a
7 TRCN0000014196 CCACATCACTTTACCTGGCTA pLKO.1 1935 CDS 100% 2.640 1.848 N PTH2R n/a
8 TRCN0000014197 GCCAATTATTCAGACTGCCTT pLKO.1 829 CDS 100% 2.640 1.848 N PTH2R n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001309516.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01338 pDONR223 100% 79.8% 79.8% None 0_1ins333 n/a
2 ccsbBroad304_01338 pLX_304 0% 79.8% 79.8% V5 0_1ins333 n/a
3 TRCN0000472969 GTTCGGTCTCAGATTGTGCCTACC pLX_317 30.8% 79.8% 79.8% V5 0_1ins333 n/a
4 TRCN0000489335 CAGATAGACCCAGCGGTGCGCTAT pLX_317 19.6% 79.8% 79.8% V5 0_1ins333 n/a
5 TRCN0000489106 ATACTAGGCTGGCTGGTCGGCCTC pLX_317 24.5% 79.8% 79.8% V5 (not translated due to prior stop codon) 0_1ins333 n/a
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