Construct: ORF TRCN0000472975
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004321.1_s317c1
- Derived from:
- ccsbBroadEn_06540
- DNA Barcode:
- TCGTCCATGACCTTTGTCAACCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LTA (4049)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472975
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4049 | LTA | lymphotoxin alpha | NM_000595.4 | 99.8% | 99.5% | 37T>C |
| 2 | human | 4049 | LTA | lymphotoxin alpha | NM_001159740.2 | 99.8% | 99.5% | 37T>C |
| 3 | human | 4049 | LTA | lymphotoxin alpha | XM_011514615.2 | 99.8% | 99.5% | 37T>C |
| 4 | human | 4049 | LTA | lymphotoxin alpha | XM_011514616.2 | 99.8% | 99.5% | 37T>C |
| 5 | human | 4049 | LTA | lymphotoxin alpha | XM_011514617.2 | 99.8% | 99.5% | 37T>C |
| 6 | human | 4049 | LTA | lymphotoxin alpha | XM_011514618.1 | 99.8% | 99.5% | 37T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 681
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac accacctgaa cgtctcttcc tcccaagggt gcgtggcacc accctacacc 121 tcctccttct ggggctgctg ctggttctgc tgcctggggc ccaggggctc cctggtgttg 181 gcctcacacc ttcagctgcc cagactgccc gtcagcaccc caagatgcat cttgcccaca 241 gcaccctcaa acctgctgct cacctcattG GAGACCCCAG CAAGCAGAAC TCACTGCTCT 301 GGAGAGCAAA CACGGACCGT GCCTTCCTCC AGGATGGTTT CTCCTTGAGC AACAATTCTC 361 TCCTGGTCCC CACCAGTGGC ATCTACTTCG TCTACTCCCA GGTGGTCTTC TCTGGGAAAG 421 CCTACTCTCC CAAGGCCACC TCCTCCCCAC TCTACCTGGC CCATGAGGTC CAGCTCTTCT 481 CCTCCCAGTA CCCCTTCCAT GTGCCTCTCC TCAGCTCCCA GAAGATGGTG TATCCAGGGC 541 TGCAGGAACC CTGGCTGCAC TCGATGTACC ACGGGGCTGC GTTCCAGCTC ACCCAGGGAG 601 ACCAGCTATC CACCCACACA GATGGCATCC CCCACCTAGT CCTCAGCCCT AGTACTGTCT 661 TCTTTGGAGC CTTCGCTCTG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCGT CCATGACCTT 841 TGTCAACCAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt