Construct: ORF TRCN0000472985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000321.1_s317c1
- Derived from:
- ccsbBroadEn_16099
- DNA Barcode:
- TTTAAGATTTCTGGGCTTCCCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABHD12B (145447)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145447 | ABHD12B | abhydrolase domain containi... | NM_181533.3 | 44.7% | 44.7% | 1_423del |
| 2 | human | 145447 | ABHD12B | abhydrolase domain containi... | NM_181814.1 | 40% | 40% | 1_513del |
| 3 | human | 145447 | ABHD12B | abhydrolase domain containi... | XM_011536474.2 | 35.6% | 35.6% | 1_618del |
| 4 | human | 145447 | ABHD12B | abhydrolase domain containi... | NM_001206673.2 | 31.4% | 31.4% | 1_744del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 432
- ORF length:
- 342
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTGGGTTGC AAGTATCAAT TATCCCTTGT 121 TAAAGATTTA CCGGAACATT CCAGGATTTT TACGTACACT TATGGATGCC CTGAGAAAAG 181 ACAAAATAAT CTTTCCTAAT GATGAAAATG TTAAATTCCT TTCTTCTCCT CTTCTCATCT 241 TACATGGAGA GGATGACAGG ACAGTGCCTT TGGAGTATGG GAAAAAGCTC TATGAAATTG 301 CACGCAATGC ATACAGGAAC AAAGAGAGGG TCAAGATGGT TATCTTTCCT CCTGGCTTCC 361 AACACAACCT GCTTTGTAAA AGCCCCACAC TGTTAATAAC CGTGAGAGAT TTCCTGAGCA 421 AGCAGTGGTC ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 481 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 541 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT AAGATTTCTG GGCTTCCCCC 601 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t