Construct: ORF TRCN0000472991
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002650.1_s317c1
- Derived from:
- ccsbBroadEn_12757
- DNA Barcode:
- TAAAAGGATCACTCTCTGCAGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMTC1 (83857)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472991
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | XM_017020007.2 | 36.7% | 36.5% | 1_1164del;1223_1231delGGTACAAGC |
| 2 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | XM_024449218.1 | 32.1% | 32% | 1_1425del;1484_1492delGGTACAAGC |
| 3 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | NM_175861.3 | 29.3% | 29.1% | 1_1632del;1691_1699delGGTACAAGC |
| 4 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | XM_017020005.1 | 27.9% | 27.7% | 1_1749del;1808_1816delGGTACAAGC |
| 5 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | XM_017020004.1 | 25.9% | 25.8% | 1_1935del;1994_2002delGGTACAAGC |
| 6 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | NM_001193451.2 | 25.7% | 25.6% | 1_1956del;2015_2023delGGTACAAGC |
| 7 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | XM_017020003.1 | 25.5% | 25.4% | 1_1974del;2033_2041delGGTACAAGC |
| 8 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | NM_001367875.2 | 24% | 23.9% | 1_2142del;2201_2209delGGTACAAGC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 747
- ORF length:
- 681
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaacttggga agactctaca ggtcactggg agagaacagc atggctgaag 121 aatgcgccct gcaggtggca cacaaagctg agatattgtc acctttggga gcactgtatt 181 acaacactgg ccgatacgaa gaggctttgc agatttacca ggaagctgca gcacttcagc 241 cttctcagag ggagctccgc ttggcactgg ctcaggtttt ggccgtgatg ggTCAGACAA 301 AAGAAGCTGA AAAGATGACC AATCACATTG TGTCAGAGGA GACCGGATGC CTTGAATGCT 361 ATCGCCTCTT GTCAGCCATC TATAGCAAGC AGGAGAACCA CGACAAGGCA CTTGATGCTA 421 TAGACAAGGC TCTCCAGCTG AAACCAAAGG ACCCAAAAGT CATTTCTGAA CTTTTTTTCA 481 CAAAAGGAAA CCAATTAAGA GAGCAGAACC TTCTCGACAA AGCTTTTGAG AGCTATAGAG 541 TGGCTGTGCA ACTAAACCCA GACCAAGCAC AGGCCTGGAT GAACATGGGT GGCATCCAAC 601 ACATCAAGGG AAAATATGTG TCTGCAAGAG CTTATTATGA GAGAGCCTTA CAGCTGGTTC 661 CAGACAGCAA ACTGCTGAAG GAAAATCTTG CCAAATTGGA TCGCCTAGAA AAACGATTAC 721 AAGAAGTTCG AGAAAAGGAT CAAACATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 781 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 841 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATAAAAGGA 901 TCACTCTCTG CAGGACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 961 aagatt