Construct: ORF TRCN0000473018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005329.1_s317c1
- Derived from:
- ccsbBroadEn_13911
- DNA Barcode:
- GGCCCAACTCCAAGCACAAAAAAG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA9 (4704)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473018
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4704 | NDUFA9 | NADH:ubiquinone oxidoreduct... | NM_005002.5 | 99.8% | 99.2% | 1115C>T;1125delC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1197
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctgccgca caatcccggg ttgtccgggt cctgtcaatg tcacgttctg 121 ccattactgc aatagccaca tctgtgtgtc acggcccacc ctgtcgccag cttcatcatg 181 ccctcatgcc tcatgggaaa ggtggacgtt cctcagtcag tgggattgtg gccactgtgt 241 ttggagcaac aggattcctg gggcgatatg ttgtcaacca ccttggacgc atggggtcac 301 aggtaatcat accctatcgg tgtgataaat atgacatcat gcaccttcgt cccatgggtg 361 acctgggcca gcttctgttt ctggaatggg acgcgagaga taaagattct atccgacgag 421 tagtacaaca cagcaatgtg gtcatcaatc ttattggacg agactgggaa accaaaaact 481 ttgattttga ggatgttttt gtgaagattc cccaagcaat tgctcaactg tccaaggaag 541 ctggagttga aaaattcatt catgtttcac atctgaatgc gaatattaaa agctcttcta 601 gatatttgag aaataaggct gttggagaga aagtagtgag agatgcattt ccggaagcca 661 ttatcgtaaa gccgtcggac atctttggaa gagaggatag attccttaat TCTTTTGCAA 721 GTATGCATCG GTTTGGTCCT ATACCCCTTG GTTCCTTGGG CTGGAAGACA GTTAAACAAC 781 CAGTATATGT CGTAGATGTA TCCAAAGGAA TTGTTAATGC AGTTAAGGAT CCTGATGCCA 841 ATGGGAAATC CTTTGCTTTC GTTGGTCCCA GTCGGTACCT CCTTTTCCAC CTGGTGAAGT 901 ACATCTTTGC TGTGGCTCAC AGATTGTTCC TCCCATTCCC CTTGCCGCTT TTTGCCTATC 961 GATGGGTAGC AAGAGTCTTT GAAATAAGCC CATTTGAGCC CTGGATAACA AGGGATAAAG 1021 TGGAGCGGAT GCACATCACA GACATGAAAT TGCCTCACCT GCCTGGCTTA GAAGACCTTG 1081 GTATTCAGGC AACACCACTG GAACTCAAGG CCATTGAGGT GCTGCGGCGT CATCGCACTT 1141 ACCGCTGGCT GTCTGCTGAA ATTGAGGATG TGAAGCCGGT CAAGACCGTA ACATTTACCC 1201 AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 1261 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 1321 TATATCTTGT GGAAAGGACG AGGCCCAACT CCAAGCACAA AAAAGACGCG TTAAGTCgac 1381 aatcaacctc tggattacaa aatttgtgaa agatt