Construct: ORF TRCN0000473059
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011067.1_s317c1
- Derived from:
- ccsbBroadEn_08214
- DNA Barcode:
- TCATAAGGATCCTAGGCTCATATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAL (51083)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473059
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51083 | GAL | galanin and GMAP prepropeptide | NM_015973.5 | 99.7% | 100% | 24G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 459
- ORF length:
- 369
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCCGAGG CAGCGCCCTC CTTCTCGCCT 121 CCCTCCTCCT CGCCGCGGCC CTTTCTGCCT CTGCGGGGCT CTGGTCGCCG GCCAAGGAAA 181 AACGAGGCTG GACCCTGAAC AGCGCGGGCT ACCTGCTGGG CCCACATGCC GTTGGCAACC 241 ACAGGTCATT CAGCGACAAG AATGGCCTCA CCAGCAAGCG GGAGCTGCGG CCCGAAGATG 301 ACATGAAACC AGGAAGCTTT GACAGGTCCA TACCTGAAAA CAATATCATG CGCACAATCA 361 TTGAGTTTCT GTCTTTCTTG CATCTCAAAG AGGCCGGTGC CCTCGACCGC CTCCTGGATC 421 TCCCCGCCGC AGCCTCCTCA GAAGACATCG AGCGGTCCTG CCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGATCATAA GGATCCTAGG CTCATATCAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt