Construct: ORF TRCN0000473061
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006777.3_s317c1
- Derived from:
- ccsbBroadEn_14894
- DNA Barcode:
- ATCAGCGTATACCTCCCCGCCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYRK3 (8444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473061
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8444 | DYRK3 | dual specificity tyrosine p... | NM_001004023.2 | 99.9% | 99.8% | 878G>A |
| 2 | human | 8444 | DYRK3 | dual specificity tyrosine p... | XM_005273315.4 | 99.9% | 99.8% | 878G>A |
| 3 | human | 8444 | DYRK3 | dual specificity tyrosine p... | XM_011510061.2 | 97.3% | 97.1% | 0_1ins45;833G>A |
| 4 | human | 8444 | DYRK3 | dual specificity tyrosine p... | NM_003582.3 | 95.5% | 95.4% | (many diffs) |
| 5 | mouse | 226419 | Dyrk3 | dual-specificity tyrosine-(... | XM_006529427.2 | 82.5% | 87.3% | (many diffs) |
| 6 | mouse | 226419 | Dyrk3 | dual-specificity tyrosine-(... | NM_145508.2 | 81.2% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1770
- ORF length:
- 1704
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtggaaagag aagttggggg atggtgtcta tgacaccttc atgatgatag 121 atgaaaccaa atgtcccccc tgttcaaatg tactctgcaa tccttctgaa ccacctccac 181 ccagaagact aaatatgacc actgagcagt ttacaggaga tcatactcag cactttttgg 241 atggaggtga gatgaaggta gaacagctgt ttcaagaatt tggcaacaga aaatccaata 301 ctattcagtc agatggcatc agtgactctg aaaaatgctc tcctactgtt tctcagggta 361 aaagttcaga ttgcttgaat acagtaaaat ccaacagttc atccaaggca cccaaagtgg 421 tgcctctgac tccagaacaa gccctgaagc aatataaaca ccacctcact gcctatgaga 481 aactggaaat aattaattat ccagaaattt actttgtagg tccaaatgcc aagaaaagac 541 atggagttat tggtggtccc aataatggag ggtatgatga tgcagatggg gcctatattc 601 atgtacctcg agaccatcta gcttatcgat atgaggtgct gaaaattatt ggcaagggga 661 gttttgggca ggtggccagg gtctatgatc acaaacttcg acagtacgtg gccctaaaaa 721 tggtgcgcaa tgagaagcgc tttcatcgtc aagcagctga ggagatccgg attttggagc 781 atcttaagaa acaggataaa actggtagta tgaacgttat ccacatgctg gaaagtttca 841 cattccggaa ccatgtttgc atggcctttg aattgctgag catagacctt tatgagctga 901 ttaaaaaaaa taagtttcag ggttttagcg tccagttggt acacaagttt gcccagtcca 961 tcttgcaatc tttggatgcc ctccacaaaa ataagattat tcactgcgat ctgaagccag 1021 aaaacattct cctgaaacac cacgggcgca gttcaaccaa ggtcattgac tttgggtcca 1081 gctgtttcga gtaccagaag ctctacacat atatccagtc tcggttctac agagctccag 1141 aaatcatctt aggaagccgc tacagcacac caattgacat atggagtttt ggctgcatcc 1201 ttgcagaact tttaacagga cagcctctct tccctggaga ggatgaagga gaccagttgg 1261 cctgcatgat ggagcttcta gggatgccac caccaaaact tctggagcaa tccaaacgtg 1321 ccaagtactt tattaattcc aagggcatac cccgctactg cTCTGTGACT ACCCAGGCAG 1381 ATGGGAGGGT TGTGCTTGTG GGGGGTCGCT CACGTAGGGG TAAAAAGCGG GGTCCCCCAG 1441 GCAGCAAAGA CTGGGGGACA GCACTGAAAG GGTGTGATGA CTACTTGTTT ATAGAGTTCT 1501 TGAAAAGGTG TCTTCACTGG GACCCCTCTG CCCGCTTGAC CCCAGCTCAA GCATTAAGAC 1561 ACCCTTGGAT TAGCAAGTCT GTCCCCAGAC CTCTCACCAC CATAGACAAG GTGTCAGGGA 1621 AACGGGTAGT TAATCCTGCA AGTGCTTTCC AGGGATTGGG TTCTAAGCTG CCTCCAGTTG 1681 TTGGAATAGC CAATAAGCTT AAAGCTAACT TAATGTCAGA AACCAATGGT AGTATACCCC 1741 TATGCAGTGT ATTGCCAAAA CTGATTAGCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1801 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1861 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATCAG 1921 CGTATACCTC CCCGCCGTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1981 tgaaagatt