Construct: ORF TRCN0000473069
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005873.1_s317c1
- Derived from:
- ccsbBroadEn_01550
- DNA Barcode:
- TTGCATTGTTTTAACTCCCAGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC18A2 (6571)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473069
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6571 | SLC18A2 | solute carrier family 18 me... | NM_003054.6 | 100% | 100% | |
| 2 | mouse | 214084 | Slc18a2 | solute carrier family 18 (v... | NM_172523.3 | 88.3% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1611
- ORF length:
- 1542
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccctgagc gagctggcgc tggtccgctg gctgcaggag agccgccgct 121 cgcggaagct catcctgttc atcgtgttcc tggcgctgct gctggacaac atgctgctca 181 ctgtcgtggt ccccatcatc ccaagttatc tgtacagcat taagcatgag aagaatgcta 241 cagaaatcca gacggccagg ccagtgcaca ctgcctccat ctcagacagc ttccagagca 301 tcttctccta ttatgataac tcgactatgg tcaccgggaa tgctaccaga gacctgacac 361 ttcatcagac cgccacacag cacatggtga ccaacgcgtc cgctgttcct tccgactgtc 421 ccagtgaaga caaagacctc ctgaatgaaa acgtgcaagt tggtctgttg tttgcctcga 481 aagccaccgt ccagctcatc accaaccctt tcataggact actgaccaac agaattggct 541 atccaattcc catatttgcg ggattctgca tcatgtttgt ctcaacaatt atgtttgcct 601 tctccagcag ctatgccttc ctgctgattg ccaggtcgct gcagggcatc ggctcgtcct 661 gctcctctgt ggctgggatg ggcatgcttg ccagtgtcta cacagatgat gaagagagag 721 gcaacgtcat gggaatcgcc ttgggaggcc tggccatggg ggtcttagtg ggccccccct 781 tcgggagtgt gctctatgag tttgtgggga agacggctcc gttcctggtg ctggccgccc 841 tggtactctt ggatggagct attcagctct ttgtgctcca gccgtcccgg gtgcagccag 901 agagtcagaa ggggacaccc ctaaccacgc tgctgaagga cccgtacatc ctcattgctg 961 caggctccat ctgctttgca aacatgggca tcgccatgct ggagccagcc ctgcccatct 1021 ggatgatgga gaccatgtgt tcccgaaagt ggcagctggg cgttgccttc ttgccagcta 1081 gtatctctta tctcattGGA ACCAATATTT TTGGGATACT TGCACACAAA ATGGGGAGGT 1141 GGCTTTGTGC TCTTCTGGGA ATGATAATTG TTGGAGTCAG CATTTTATGT ATTCCATTTG 1201 CAAAAAACAT TTATGGACTC ATAGCTCCGA ACTTTGGAGT TGGTTTTGCA ATTGGAATGG 1261 TGGATTCGTC AATGATGCCT ATCATGGGCT ACCTCGTAGA CCTGCGGCAC GTGTCCGTCT 1321 ATGGGAGTGT GTACGCCATT GCGGATGTGG CATTTTGTAT GGGGTATGCT ATAGGTCCTT 1381 CTGCTGGTGG TGCTATTGCA AAGGCAATTG GATTTCCATG GCTCATGACA ATTATTGGGA 1441 TAATTGATAT TCTTTTTGCC CCTCTCTGCT TTTTTCTTCG AAGTCCACCT GCCAAAGAAG 1501 AAAAAATGGC TATTCTCATG GATCACAACT GCCCTATTAA AACAAAAATG TACACTCAGA 1561 ATAATATCCA GTCATATCCG ATAGGTGAAG ATGAAGAATC TGAAAGTGAC TTGCCAACTT 1621 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1681 CTACGTAGTA ATGAACTAGC CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1741 CTTGTGGAAA GGACGATTGC ATTGTTTTAA CTCCCAGTAC ACGCGTTAAG TCgacaatca 1801 acctctggat tacaaaattt gtgaaagatt