Construct: ORF TRCN0000473071
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013462.1_s317c1
- Derived from:
- ccsbBroadEn_04925
- DNA Barcode:
- CCAATAACCCCTAGGACCACCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PWWP3B (139221)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473071
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139221 | PWWP3B | PWWP domain containing 3B | NM_001171020.2 | 100% | 100% | |
| 2 | human | 139221 | PWWP3B | PWWP domain containing 3B | NM_152423.4 | 100% | 100% | |
| 3 | human | 139221 | PWWP3B | PWWP domain containing 3B | XM_005262077.2 | 100% | 100% | |
| 4 | human | 139221 | PWWP3B | PWWP domain containing 3B | XM_011530856.1 | 100% | 100% | |
| 5 | human | 139221 | PWWP3B | PWWP domain containing 3B | XM_011530857.1 | 100% | 100% | |
| 6 | human | 139221 | PWWP3B | PWWP domain containing 3B | XM_017029269.1 | 100% | 100% | |
| 7 | human | 139221 | PWWP3B | PWWP domain containing 3B | XM_024452334.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2157
- ORF length:
- 2088
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtctgag tatgtcctat gcaactggaa agaccagttg tggccagcaa 121 aagttttgtc cagatctgaa acttcatcaa acagtaagag gaaaaaggca ttttctctag 181 aagttcaaat actctcacta gatgaaaaaa ttaaattgga cagcacagaa acaaagatcc 241 taaataaatc tcaaattgaa gccattgctg cctcattagg actacagtca gaggacagtg 301 ctccacctac agaggaaact gcctatggaa gatcactaaa agtggcactg ggtattctga 361 atgagagaac aaatttgagt caagcaagca cttcagatga agaggagatc actatgctgt 421 ctcaaaatgt accacaaaaa cagtccgatt caccccctca taaaaaatac cggaaggatg 481 aaggtgactt accagggtgt cttgaggaaa gggaaaactc agcatgcttg ttagcatctt 541 cagagagtga tgattccctg tatgatgata aatcacaagc acccacaatg gtcgatacta 601 ttccaagtga agtggaaaca aagtcattac aaaactctag ctggtgcgag actttccctt 661 cactttcgga agataatgat gaaaaagaga acaagaataa gattgatatc tcagcagtta 721 tgtctgtgca ttctgcagtc aaagaggaaa gtgcatgtgt taaagatgaa aagtttgctc 781 cacctttgtc acctttgtca tcagatatgc tcattatgcc caaagctttg aaagaagaga 841 gcgaggatac ctgcctagag accctggctg ttccctctga atgctctgct ttctcagaga 901 atattgagga tcctggagag ggtccctcaa atccatgctt agataccagc cagaatcaac 961 cttccatgga atcagagatg ggggctgcag catgccctgg gagttgttca agggaatgcg 1021 aggtttcatt tagtgcctct aaccctgtct gggattattc acatcttatg agtagtgaaa 1081 gaaattttca gagactggat tttgaagaac ttgaggaaga aggtcaagcc tctgacaagt 1141 cattgcttcc aagtcgcatt aatctttctc tattagatga tgatgaggaa gacgaagaac 1201 ttccacgctt cattttacat tatgagacac atccgtttga aacaggaatg atagtctggt 1261 ttaaatatca gaaatatcca ttttggccag cagtgataaa aagtatcaga cgaaaagaga 1321 ggaaagcaag tgtgcttttt gttgaggcaa acatgaattc tgaaaagaag ggcattagag 1381 taaattttag aagattaaag aaatttgatt gtaaagagaa acaaatgcta gtggacaaag 1441 ccagggagga ttatagtgag agtattgact ggtgcatctc actaatttgt gactacagag 1501 ttagaatagg ttgtggttct ttcacgggct ctttgcttga gtattatgct gctgatatta 1561 gttacccagt taggaaagaa acaaaacagg atactttcag gaacaaattt ccaaagctgc 1621 ataatgaaga tgccagggaa ccgatggctg taacttccca gaccaagaaa atgtccttcc 1681 aaaaaattct ccctgaccgg atgaaggctg ctcgggaccg agccaacaag aacctggtgg 1741 acttcattgt gaatgcaaag ggaacagaga accatcTTCT GGCCATTGTA AATGGCACAA 1801 AAGGATCCAG ATGGCTGAAA TCATTTTTGA ATGCAAATAG GTTCACACCC TGTATTGAAA 1861 CATACTTTGA GGATGAAGAT CAGTTGGATG AAGTGGTGAA ATATTTACAA GAAGTCTGCA 1921 ATCAAATAGA TCAAATAATG CCAACTTGGA TAAAAGATGA TAAAATTAAA TTTATCCTAG 1981 AAGTTCTTCT GCCAGAAGCA ATTATTTGTT CAATTTCTGC TGTTGATGGG TTAGATTACG 2041 AGGCAGCTGA AGCAAAGTAT CTAAAAGGAC CATGTCTAGG CTACAGGGAA AGAGAATTAT 2101 TTGATGCAAA AATAATATAT GAAAAGAGAC GAAAAGCACC AACAAATGAA GCTCACTTGC 2161 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2221 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2281 ATATATCTTG TGGAAAGGAC GACCAATAAC CCCTAGGACC ACCTCTACGC GTTAAGTCga 2341 caatcaacct ctggattaca aaatttgtga aagatt