Construct: ORF TRCN0000473083
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009836.1_s317c1
- Derived from:
- ccsbBroadEn_13371
- DNA Barcode:
- CTATGCTTCTACGCGATTGGTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EEF1DP3 (196549)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473083
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001130055.3 | 42.9% | 30.3% | (many diffs) |
| 2 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001130057.3 | 42.9% | 30.3% | (many diffs) |
| 3 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001289950.3 | 42.9% | 30.3% | (many diffs) |
| 4 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001960.6 | 42.9% | 30.3% | (many diffs) |
| 5 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716525.1 | 42.9% | 30.3% | (many diffs) |
| 6 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001195203.3 | 42.3% | 29.6% | (many diffs) |
| 7 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001130056.4 | 36% | 23.8% | (many diffs) |
| 8 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001317743.3 | 36% | 23.8% | (many diffs) |
| 9 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001330646.2 | 36% | 23.8% | (many diffs) |
| 10 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716519.4 | 18.6% | 13.3% | (many diffs) |
| 11 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_017013171.1 | 18.6% | 13.3% | (many diffs) |
| 12 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_024447088.1 | 18.6% | 13.3% | (many diffs) |
| 13 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001130053.4 | 18.6% | 13.3% | (many diffs) |
| 14 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_032378.6 | 18.6% | 13.3% | (many diffs) |
| 15 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_005250820.4 | 18.6% | 13.3% | (many diffs) |
| 16 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_005250823.1 | 18.6% | 13.3% | (many diffs) |
| 17 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716522.1 | 18.6% | 13.3% | (many diffs) |
| 18 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716523.2 | 18.6% | 13.3% | (many diffs) |
| 19 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716524.1 | 18.6% | 13.3% | (many diffs) |
| 20 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_011516905.2 | 18.6% | 13.3% | (many diffs) |
| 21 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_011516906.3 | 18.6% | 13.3% | (many diffs) |
| 22 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_017013170.2 | 18.6% | 13.3% | (many diffs) |
| 23 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716520.4 | 15.6% | 10.4% | (many diffs) |
| 24 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_017013172.1 | 15.6% | 10.4% | (many diffs) |
| 25 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_024447089.1 | 15.6% | 10.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 465
- ORF length:
- 399
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tacaaacttt ctagtaggtg agaagatctg gttcCACAAG TTCAAATATG 121 GCGATGCAGA AAGGAGATTC TACGAACAGA TGAACGGGCC TGTGGCCGGC GCCTCCCTCC 181 AGGAGGCCAG CATGATCCTC CATGATATTG CCAGAGCCAG AGAGAACATC CCGAAATCCC 241 TGGCCGGAAG CTTAGGCCCA GGGGCGTCTA GCGGCCCCAG CGGAGACCAC AGCGAGCTCG 301 TCGTCCGGAT CGCCAGTCTG GAAGTGGACA ACCAGAGAGA CCTGGCTGAA CGTGCTGGAG 361 AAGAGCTTGC CCGGCCACTG GGCCACAGCC CCGCAGACCC AGCACATGTC TCCCATGCGC 421 CAAGTGGAGC CCCCGGCCAA GAAGCTAGCC ACACCAGCAG AGGATGCCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA CTATGCTTCT ACGCGATTGG TTGCACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt