Construct: ORF TRCN0000473093
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011247.1_s317c1
- Derived from:
- ccsbBroadEn_00377
- DNA Barcode:
- AGGCCGGGGACCTGGGGGATTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSN2 (1447)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473093
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1447 | CSN2 | casein beta | NM_001891.3 | 100% | 100% | |
| 2 | human | 1447 | CSN2 | casein beta | XM_017007760.1 | 100% | 100% | |
| 3 | human | 1447 | CSN2 | casein beta | NM_001302770.1 | 99.5% | 99.5% | 97_98insAGC |
| 4 | human | 1447 | CSN2 | casein beta | XR_001756926.1 | 10.2% | 1_94del;173_174ins599 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 747
- ORF length:
- 678
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaggtcctc atcctcgcct gcctggtggc tcttgctctt gcaagggaga 121 ccatagaaag cctttcaagc agtgaggaat ctattacaga atacaagcag aaagttgaga 181 aggttaaaca tgaggaccag cagcaaggag aggatgaaca ccaggataaa atctacccct 241 ctttccagcc acagcctctg atctatccat tcgttgaacc tatcccctat ggttttcttc 301 cacaaaacat tctgccTCTT GCTCAGCCTG CTGTGGTGCT GCCTGTCCCT CAGCCTGAAA 361 TAATGGAAGT CCCTAAAGCT AAAGACACTG TCTACACTAA GGGCAGAGTG ATGCCTGTCC 421 TTAAATCTCC AACGATACCC TTTTTTGACC CTCAAATCCC AAAACTCACT GATCTTGAAA 481 ATCTGCATCT TCCTCTGCCT CTGCTCCAGC CCTTGATGCA GCAGGTCCCT CAGCCTATTC 541 CTCAGACTCT TGCACTTCCC CCTCAGCCCC TGTGGTCTGT TCCTCAGCCC AAAGTCCTGC 601 CTATCCCCCA GCAAGTGGTG CCCTACCCTC AGAGAGCTGT GCCTGTTCAA GCCCTTCTGC 661 TCAACCAAGA ACTTCTACTT AACCCCACCC ACCAGATCTA CCCTGTGACT CAGCCACTTG 721 CCCCAGTTCA TAACCCCATT AGTGTCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 781 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 841 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGGCCGGG 901 GACCTGGGGG ATTATAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 961 aagatt