Construct: ORF TRCN0000473094
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009119.1_s317c1
- Derived from:
- ccsbBroadEn_07786
- DNA Barcode:
- CTGTTAGCATGATCTAGTTGAGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NXPH2 (11249)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473094
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11249 | NXPH2 | neurexophilin 2 | NM_007226.3 | 99.7% | 99.6% | 219C>T;429T>A |
| 2 | mouse | 18232 | Nxph2 | neurexophilin 2 | NM_008752.2 | 84.3% | 88.6% | (many diffs) |
| 3 | mouse | 18232 | Nxph2 | neurexophilin 2 | XM_011239035.2 | 79% | 82.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 861
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgcctgcgg ccgctgcccc tcgtggtggt ccctggcttg ctgcagctgc 121 tattttgtga cagtaaggaa gtggtgcatg ccacggaggg gctggattgg gaagacaaag 181 atgctccagg gacgttggtc ggcaacgtgg tgcactcaag gatcatcagt cccctgcgcc 241 tgtttgttaa acagtctccg gtgcccaagc ccggccccat ggcgtatgca gacagcatgg 301 aaaacttttg ggattggctg gccaacatca cggagattca ggagccattg gCAAGAACTA 361 AACGGAGGCC AATAGTAAAA ACAGGAAAAT TTAAGAAAAT GTTTGGATGG GGTGACTTTC 421 ATTCCAACAT TAAAACTGTC AAACTCAATC TCCTCATCAC AGGGAAAATT GTTGACCATG 481 GAAATGGAAC CTTCAGAGTG TATTTCCGAC ATAATTCAAC AGGCCTGGGC AATGTTTCAG 541 TGAGCTTGGT ACCACCCTCC AAGGTGGTGG AATTTGAAGT TTCCCCCCAG TCTACCTTGG 601 AGACCAAGGA ATCCAAATCT TTCAATTGTC GCATTGAGTA TGAAAAAACA GATCGGGCGA 661 AAAAGACCGC CCTGTGCAAC TTTGACCCAT CCAAGATCTG CTACCAGGAG CAGACTCAGA 721 GCCATGTGTC TTGGTTGTGC TCCAAGCCCT TCAAGGTCAT TTGCATTTAC ATTGCCTTTT 781 ACAGTGTTGA TTATAAACTC GTGCAAAAGG TGTGCCCTGA CTACAATTAC CATAGTGAGA 841 CCCCATACTT ATCTTCTGGC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 901 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 961 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTGT TAGCATGATC 1021 TAGTTGAGTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt