Construct: ORF TRCN0000473100
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007432.1_s317c1
- Derived from:
- ccsbBroadEn_05693
- DNA Barcode:
- AAAGATTGTAGTGCCATTGTCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COA5 (493753)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473100
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 493753 | COA5 | cytochrome c oxidase assemb... | NM_001008215.3 | 100% | 100% | |
| 2 | mouse | 76178 | Coa5 | cytochrome C oxidase assemb... | NM_198006.4 | 85.5% | 82.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 277
- ORF end:
- 499
- ORF length:
- 222
- Sequence:
-
1 CCAGGCACCT CGATTAGTTC TCGAGCTTTT GGAGTACGTC GTCTTTAGGT TGGGGGGAGG 61 GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT AGGCCAGCTT 121 GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC 181 TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGCTAGC 241 ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCATGC CTAAGTATTA TGAGGACAAG 301 CCGCAGGGCG GCGCGTGCGC GGGCCTGAAG GAGGACCTGG GCGCGTGTCT GCTGCAGTCG 361 GACTGTGTGG TCCAGGAAGG AAAATCACCT CGGCAGTGTT TGAAGGAAGG ATACTGCAAC 421 TCTTTGAAGT ACGCATTTTT TGAGTGTAAA AGATCAGTGT TGGATAACAG GGCAAGATTC 481 AGAGGAAGAA AAGGATATTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT 541 ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA 601 AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAAGAT TGTAGTGCCA 661 TTGTCTTTAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt