Construct: ORF TRCN0000473110
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001784.1_s317c1
- Derived from:
- ccsbBroadEn_02608
- DNA Barcode:
- GCACTGCCGGCGAATTGCCCAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WWP2 (11060)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473110
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11060 | WWP2 | WW domain containing E3 ubi... | NM_001270455.2 | 100% | 100% | |
| 2 | human | 11060 | WWP2 | WW domain containing E3 ubi... | NM_001270454.2 | 38.4% | 38.5% | 1005_2610delinsG |
| 3 | human | 11060 | WWP2 | WW domain containing E3 ubi... | NM_007014.5 | 38.4% | 38.5% | 1005_2610delinsG |
| 4 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_011522823.2 | 38.4% | 38.5% | 1005_2610delinsG |
| 5 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_011522825.1 | 38.4% | 38.5% | 1005_2610delinsG |
| 6 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_017022879.1 | 38.4% | 38.5% | 1005_2610delinsG |
| 7 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_017022880.1 | 38.4% | 38.5% | 1005_2610delinsG |
| 8 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_017022881.1 | 38.4% | 38.5% | 1005_2610delinsG |
| 9 | human | 11060 | WWP2 | WW domain containing E3 ubi... | NM_001270453.1 | 25.1% | 25.1% | 0_1ins348;657_2262delinsG |
| 10 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_011522826.3 | 25.1% | 25.1% | 0_1ins348;657_2262delinsG |
| 11 | mouse | 66894 | Wwp2 | WW domain containing E3 ubi... | XM_017312941.1 | 87.5% | 91.6% | (many diffs) |
| 12 | mouse | 66894 | Wwp2 | WW domain containing E3 ubi... | XR_001778461.1 | 36.7% | (many diffs) | |
| 13 | mouse | 66894 | Wwp2 | WW domain containing E3 ubi... | NM_025830.3 | 33.7% | 35.2% | (many diffs) |
| 14 | mouse | 66894 | Wwp2 | WW domain containing E3 ubi... | XM_006531299.3 | 33.7% | 35.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1071
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atctgccagc tctagccggg caggagtggc cctgcctttt gagaagtctc 121 agctcacttt gaaagtggtg tccgcaaagc ccaaggtgca taatcgtcaa cctcgaatta 181 actcctacgt ggaggtggcg gtggatggac tccccagtga gaccaagaag actgggaagc 241 gcattgggag ctctgagctt ctctggaatg agatcatcat tttgaatgtc acggcacaga 301 gtcatttaga tttaaaggtc tggagctgcc ataccttgag aaatgaactg ctaggcaccg 361 catctgtcaa cctctccaac gtcttgaaga acaatggggg caaaatggag aacatgcagc 421 tgaccctgaa cctgcagacg gagaacaaag gcagcgttgt ctcaggcgga gagctgacaa 481 ttttcctgga cgggccaact gttgatctgg gaaatgtgcc taatggcagt gccctgacag 541 atggatcaca gctgccttcg agagactcca gtggaacagc agtagctcca gagaaccggc 601 accagccccc cagcacaaac tgctttggtg gaagatcccg gacgcacaga cattcgggtg 661 cttcagccag aaCAACCCCA GCAACCGGCG AGCAAAGCCC CGGTGCTCGG AGCCGGCACC 721 GCCAGCCCGT CAAGAACTCA GGCCACAGTG GCTTGGCCAA TGGCACAGTG AATGATGAAC 781 CCACAACAGC CACTGATCCC GAAGAACCTT CCGTTGTTGG TGTGACGTCC CCACCTGCTG 841 CACCCTTGAG TGTGACCCCG AATCCCAACA CGACTTCTCT CCCTGCCCCA GCCACACCGG 901 CTGAAGGAGA GGAACCCAGC ACTTCGGGTA CACAGCAGCT CCCAGCGGCT GCCCAGGCCC 961 CCGACGCTCT GCCTGCTGGA TGGGAACAGC GAGAGCTGCC CAACGGACGT GTCTATTATG 1021 TTGACCACAA TACCAAGACC ACCACCTGGG AGCGGCCCCT TCCTCCAGGG TACCCAACTT 1081 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1141 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1201 CTTGTGGAAA GGACGAGCAC TGCCGGCGAA TTGCCCAAAT ACGCGTTAAG TCgacaatca 1261 acctctggat tacaaaattt gtgaaagatt