Construct: ORF TRCN0000473135
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011088.1_s317c1
- Derived from:
- ccsbBroadEn_15364
- DNA Barcode:
- GAAATTTATTAACAAAGAAGGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP5PB (515)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473135
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 515 | ATP5PB | ATP synthase peripheral sta... | NM_001688.5 | 99.7% | 99.2% | 272A>G;581A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 834
- ORF length:
- 768
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtcccgggtg gtactttccg ccgccgccac agcggccccc tctctgaaga 121 atgcagcctt cctaggtcca ggggtattgc aggcaacaag gacctttcat acagggcagc 181 cacaccttgt ccctgtacca cctcttcctg aatacggagg aaaagttcgt tatggactga 241 tccctgagga attcttccag tttctttatc ctaaaactgg tgtaacagga ccctatgtac 301 tcGGAACTGG GCTTATCTTG TACGCTTTAT CCAAAGGAAT ATATGTGATT AGCGCAGAGA 361 CCTTCACTGC CCTATCAGTA CTAGGTGTAA TGGTCTATGG AATTAAAAAA TATGGTCCCT 421 TTGTTGCAGA CTTTGCTGAT AAACTCAATG AGCAAAAACT TGCCCAACTA GAAGAGGCGA 481 AGCAGGCTTC CATCCAACAC ATCCAGAATG CAATTGATAC GGAGAAGTCA CAACAGGCAC 541 TGGTTCAGAA GCGCCATTAC CTTTTTGATG TGCAAAGGAA TAACATTGCT ATGGCTTTGG 601 AAGTTACTTA CCGGGAACGA CTGTATAGAG TATATAAGGA AGTAAGGAAT CGCCTGGACT 661 ATCATATATC TGTGCAGAAC ATGATGCGTC GAAAGGAACA AGAACACATG ATAAATTGGG 721 TGGAGAAGCA CGTGGTGCAA AGCATCTCCA CACAGCAGGA AAAGGAGACA ATTGCCAAGT 781 GCATTGCGGA CCTAAAGCTG CTGGCAAAGA AGGCTCAAGC ACAGCCAGTT ATGTACCCAA 841 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 901 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 961 TATCTTGTGG AAAGGACGAG AAATTTATTA ACAAAGAAGG TTCACGCGTT AAGTCgacaa 1021 tcaacctctg gattacaaaa tttgtgaaag att