Transcript: Human NM_001688.5

Homo sapiens ATP synthase peripheral stalk-membrane subunit b (ATP5PB), mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
ATP5PB (515)
Length:
2628
CDS:
79..849

Additional Resources:

NCBI RefSeq record:
NM_001688.5
NBCI Gene record:
ATP5PB (515)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001688.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000230282 CCTATCAGTACTAGGTGTAAT pLKO_005 384 CDS 100% 13.200 18.480 N ATP5PB n/a
2 TRCN0000029368 CGGGAACGACTGTATAGAGTA pLKO.1 625 CDS 100% 4.950 6.930 N ATP5PB n/a
3 TRCN0000230284 GTCTACTGAAGTTATAGTTTA pLKO_005 936 3UTR 100% 13.200 10.560 N ATP5PB n/a
4 TRCN0000218193 CATCCAGAATGCAATTGATAC pLKO_005 513 CDS 100% 10.800 7.560 N ATP5PB n/a
5 TRCN0000029364 GCTGATAAACTCAATGAGCAA pLKO.1 448 CDS 100% 2.640 1.848 N ATP5PB n/a
6 TRCN0000029367 TCTTCCTGAATACGGAGGAAA pLKO.1 216 CDS 100% 0.495 0.347 N ATP5PB n/a
7 TRCN0000257141 GGCTTATCTTGTACGCTTTAT pLKO_005 323 CDS 100% 13.200 7.920 N ATP5PB n/a
8 TRCN0000029365 GTACGCTTTATCCAAAGAAAT pLKO.1 333 CDS 100% 13.200 7.920 N ATP5PB n/a
9 TRCN0000029366 GCAGAACATGATGCGTCGAAA pLKO.1 687 CDS 100% 4.950 2.970 N ATP5PB n/a
10 TRCN0000230283 ATCGCCTGGACTATCATATAT pLKO_005 662 CDS 100% 15.000 7.500 Y ATP5PB n/a
11 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 1531 3UTR 100% 4.050 2.025 Y P3H4 n/a
12 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 1531 3UTR 100% 4.050 2.025 Y ORAI2 n/a
13 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 1531 3UTR 100% 4.050 2.025 Y P3H4 n/a
14 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 1659 3UTR 100% 10.800 5.400 Y SMIM11A n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001688.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00130 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00130 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000473444 CGCTCCCGTCGCTTTCCGGTTGCG pLX_317 66.6% 100% 100% V5 n/a
4 ccsbBroadEn_05869 pDONR223 100% 99.8% 100% None 351T>C n/a
5 ccsbBroad304_05869 pLX_304 0% 99.8% 100% V5 351T>C n/a
6 TRCN0000473136 AGCAACCCAATCCGTAACGGCTCA pLX_317 67.8% 99.8% 100% V5 351T>C n/a
7 ccsbBroadEn_15364 pDONR223 0% 99.7% 99.2% None 272A>G;581A>G n/a
8 ccsbBroad304_15364 pLX_304 0% 99.7% 99.2% V5 272A>G;581A>G n/a
9 TRCN0000473135 GAAATTTATTAACAAAGAAGGTTC pLX_317 68.7% 99.7% 99.2% V5 272A>G;581A>G n/a
Download CSV