Construct: ORF TRCN0000473140
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016489.1_s317c1
- Derived from:
- ccsbBroadEn_12265
- DNA Barcode:
- TGGTTCCTGTATTGCCCGGGGAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENAH (55740)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473140
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55740 | ENAH | ENAH actin regulator | NM_018212.6 | 23.3% | 22.1% | (many diffs) |
| 2 | human | 55740 | ENAH | ENAH actin regulator | NM_001008493.3 | 22.5% | 21.3% | (many diffs) |
| 3 | human | 55740 | ENAH | ENAH actin regulator | XM_024448316.1 | 17.1% | 15.5% | (many diffs) |
| 4 | human | 55740 | ENAH | ENAH actin regulator | XM_024448317.1 | 17.1% | 15.5% | (many diffs) |
| 5 | human | 55740 | ENAH | ENAH actin regulator | XM_024448315.1 | 16.9% | 15.2% | (many diffs) |
| 6 | human | 55740 | ENAH | ENAH actin regulator | XM_024448314.1 | 16.7% | 15.1% | (many diffs) |
| 7 | human | 55740 | ENAH | ENAH actin regulator | XM_024448313.1 | 16.6% | 15% | (many diffs) |
| 8 | human | 55740 | ENAH | ENAH actin regulator | XM_024448311.1 | 16.4% | 14.8% | (many diffs) |
| 9 | human | 55740 | ENAH | ENAH actin regulator | XM_017001752.1 | 16.2% | 14.9% | (many diffs) |
| 10 | human | 55740 | ENAH | ENAH actin regulator | XM_024448309.1 | 16.1% | 14.6% | (many diffs) |
| 11 | human | 55740 | ENAH | ENAH actin regulator | XM_024448310.1 | 16.1% | 14.6% | (many diffs) |
| 12 | human | 55740 | ENAH | ENAH actin regulator | XM_017001751.1 | 16% | 15.2% | (many diffs) |
| 13 | human | 55740 | ENAH | ENAH actin regulator | XM_017001749.1 | 15.7% | 14.8% | (many diffs) |
| 14 | human | 55740 | ENAH | ENAH actin regulator | XM_017001748.1 | 15.6% | 14.8% | (many diffs) |
| 15 | human | 55740 | ENAH | ENAH actin regulator | XM_017001747.1 | 15.6% | 14.8% | (many diffs) |
| 16 | human | 55740 | ENAH | ENAH actin regulator | XM_017001750.1 | 15.4% | 14.2% | (many diffs) |
| 17 | human | 55740 | ENAH | ENAH actin regulator | XM_011544229.2 | 15.3% | 14.5% | (many diffs) |
| 18 | human | 55740 | ENAH | ENAH actin regulator | XM_017001746.2 | 15.2% | 14.4% | (many diffs) |
| 19 | human | 55740 | ENAH | ENAH actin regulator | XM_024448319.1 | 13% | 11.8% | (many diffs) |
| 20 | human | 55740 | ENAH | ENAH actin regulator | XM_024448318.1 | 12.8% | 11.6% | (many diffs) |
| 21 | human | 55740 | ENAH | ENAH actin regulator | XM_024448308.1 | 12.8% | 11.6% | (many diffs) |
| 22 | human | 55740 | ENAH | ENAH actin regulator | XM_024448307.1 | 12.7% | 11.5% | (many diffs) |
| 23 | human | 55740 | ENAH | ENAH actin regulator | XM_024448306.1 | 12.5% | 11.4% | (many diffs) |
| 24 | human | 55740 | ENAH | ENAH actin regulator | XM_024448305.1 | 12.5% | 11.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 91
- ORF end:
- 529
- ORF length:
- 438
- Sequence:
-
1 CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC TAGCATCGAT 61 TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGGAAATTC AAAGAAGACA ACTACAAGAA 121 CAGCAACGGC AAAAGGAGCT GGAGCGGGAA AGGCTGGAGC GAGAAAGAAT GGAAAGAGAA 181 AGGTTGGAGA GAGAGAGGTT AGAAAGGGAA AGGCTGGAGA GGGAGCGACT GGAACAAGAA 241 CAGCTGGAGA GAGAGAGACA AGAACGGGAA CGGCAGGAAC GCCTGGAGCG GCAGGAACGC 301 CTGGAGCGGC AGGAACGCCT GGATCGGGAG AGGCAAGAAA GACAAGAACG AGAGAGGCTG 361 GAGAGACTGG AACGGGAGAG GCAAGAAAGG GAGCGACAAG AGCAGTTAGA AAGGGAACAG 421 CTGGAATGGG AGAGAGAGCG CAGAATATCA AGTGCTGGTA AGAAGTTTAC AAACTCTTCT 481 GTGTTCTTGT ATCTGAGCTT GGATGATGTC TCTTGTGCAG TCATGTGTTT CCCAACTTTC 541 TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT 601 ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 661 TGTGGAAAGG ACGATGGTTC CTGTATTGCC CGGGGAAGAC GCGTTAAGTC gacaatcaac 721 ctctggatta caaaatttgt gaaagatt