Construct: ORF TRCN0000473150
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017941.1_s317c1
- Derived from:
- ccsbBroadEn_11853
- DNA Barcode:
- CAACAATCGTTTAGCGGTACACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAAA (27163)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473150
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27163 | NAAA | N-acylethanolamine acid ami... | NM_001042402.2 | 61.6% | 60.9% | 591_622del;627_632delGTTTCG;636_969del |
| 2 | human | 27163 | NAAA | N-acylethanolamine acid ami... | NM_001363719.2 | 61.6% | 60.9% | 591_622del;627_632delGTTTCG;636_969del |
| 3 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XM_011531854.3 | 61.2% | 60.6% | 591_622del;627_632delGTTTCG;636_975del |
| 4 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XM_005262924.3 | 61% | 60.4% | 591_622del;627_632delGTTTCG;636_978del |
| 5 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XM_005262923.3 | 59.7% | 59.1% | 591_622del;627_632delGTTTCG;636_999del |
| 6 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XM_017008030.2 | 59.5% | 58.9% | 591_622del;627_632delGTTTCG;636_1002del |
| 7 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XM_005262921.4 | 59.2% | 58.6% | 591_622del;627_632delGTTTCG;636_1008del |
| 8 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XM_006714180.3 | 58.5% | 57.9% | 591_622del;627_632delGTTTCG;636_1020del |
| 9 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XM_011531852.3 | 57.3% | 56.7% | 591_622del;627_632delGTTTCG;636_1041del |
| 10 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XM_017008029.2 | 55.7% | 55.1% | 591_622del;627_632delGTTTCG;636_1071del |
| 11 | human | 27163 | NAAA | N-acylethanolamine acid ami... | NM_014435.4 | 55.4% | 54.8% | 591_622del;627_632delGTTTCG;636_1077del |
| 12 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XM_005262920.4 | 55.4% | 54.8% | 591_622del;627_632delGTTTCG;636_1077del |
| 13 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XM_017008028.2 | 55.4% | 54.8% | 591_622del;627_632delGTTTCG;636_1077del |
| 14 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XM_017008027.2 | 54.3% | 53.8% | 591_622del;627_632delGTTTCG;636_1098del |
| 15 | human | 27163 | NAAA | N-acylethanolamine acid ami... | XR_001741202.2 | 32.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gaccgcggac cgggaggcgc gcccggggct tccgtccctg ctgctgctgc 121 tgctggccgg ggccgggctg tcagccgcct cgcccccagc agcgccgcgc ttcaacgtga 181 gcctggactc ggtccccgag ctgcgctggc tgcccgtgct gcggcacTAC GACTTGGACT 241 TGGTGCGCGC CGCGATGGCG CAAGTCATCG GGGACAGAGT CCCCAAGTGG GTGCACGTGT 301 TAATCGGAAA AGTGGTCCTG GAGCTGGAGC GCTTCCTGCC CCAGCCCTTC ACCGGCGAGA 361 TCCGCGGCAT GTGTGACTTC ATGAACCTCA GCCTGGCGGA CTGCCTTCTG GTCAACCTGG 421 CCTACGAGTC CTCCGTGTTC TGCACCAGTA TTGTGGCTCA AGACTCCAGA GGCCACATTT 481 ACCATGGTCG GAATTTGGAT TATCCTTTTG GGAATGTCTT ACGCAAGCTG ACAGTGGATG 541 TGCAATTCTT AAAGAATGGG CAGATTGCAT TCACAGGAAC TACTTTTATT GGCTATGTAG 601 GATTATGGAC TGGCCAGAGC CCACACAAGT TTACAGTTTC TGGTGATGAA CGAGACCCTG 661 AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGACA ACAATCGTTT AGCGGTACAC CTACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt