Construct: ORF TRCN0000473156
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013572.1_s317c1
- Derived from:
- ccsbBroadEn_08979
- DNA Barcode:
- CTTAAGAACCCACATGAATTAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPP21 (79897)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473156
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79897 | RPP21 | ribonuclease P/MRP subunit p21 | NM_024839.3 | 99.7% | 99.3% | 231G>C |
| 2 | human | 79897 | RPP21 | ribonuclease P/MRP subunit p21 | NM_001199120.2 | 94.8% | 94.4% | 158_181del;255G>C |
| 3 | human | 79897 | RPP21 | ribonuclease P/MRP subunit p21 | NM_001199121.2 | 90.4% | 75.1% | 231G>C;366_372delAGGTGAG;432_433ins37 |
| 4 | human | 202658 | TRIM39-RPP21 | TRIM39-RPP21 readthrough | NM_001199119.1 | 29.5% | 26.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 528
- ORF length:
- 462
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGGC GGGGCCGGTG AAGGACCGCG AGGCCTTCCA GAGGCTCAAC TTCCTGTACC 121 AGGCCGCCCA TTGTGTCCTT GCCCAGGACC CCGAGAACCA GGCGCTGGCG AGGTTTTACT 181 GCTACACTGA GAGGACCATT GCGAAGCGGC TCGTCTTGCG GCGGGATCCC TCGGTGAAGA 241 GGACTCTCTG TCGAGGCTGC TCTTCCCTCC TCGTCCCGGG CCTCACCTGC ACCCACCGCC 301 AGAGACGCTG CAGGGGACAG CGCTGGACCG TACAGACCTG CCTAACATGC CAGCGCAGCC 361 AACGCTTCCT CAATGATCCC GGGCATTTAC TCTGGGGAGA CAGGCCTGAG GCCCAGCTCG 421 GGAGCCAAGC AGATTCCAAA CCACTACAAC CCTTGCCAAA CACAGCCCAC TCCATTTCAG 481 ACCGCCTTCC TGAGGAGAAA ATGCAGACTC AGGGTTCCAG TAACCAGTGC CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGACTTAAGA ACCCACATGA ATTAGTTACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt