Construct: ORF TRCN0000473161
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008497.1_s317c1
- Derived from:
- ccsbBroadEn_08803
- DNA Barcode:
- AATCACTTTTCTTAATGTCTCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIIP (60672)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473161
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60672 | MIIP | migration and invasion inhi... | NM_021933.4 | 99.7% | 99.2% | 297A>T;424C>T;499A>G |
| 2 | human | 60672 | MIIP | migration and invasion inhi... | XM_011541895.1 | 99.7% | 99.2% | 297A>T;424C>T;499A>G |
| 3 | human | 60672 | MIIP | migration and invasion inhi... | XM_011541896.1 | 99.7% | 99.2% | 297A>T;424C>T;499A>G |
| 4 | human | 60672 | MIIP | migration and invasion inhi... | XM_005263487.4 | 93.5% | 92.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1230
- ORF length:
- 1164
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggaggctgag gaactggcac agctgcggct gctcaatctg gagctcctga 121 ggcagctgtg ggtggggcag gatgctgtgc ggcggtcagt ggccagggca gcctcggagt 181 caagcctgga atccagcagc agctacaact cagagactcc atcgacccca gagacgtcct 241 caacttcctt gagcacctcc tgcccacggg gccggtcctc cgtgtggggc ccaccagatg 301 cctgtcgagg ggacctccgt gatgtggcca gatcgggggt ggcctctctc ccacctgcca 361 attgccagca ccaggagtcc ctgggccgac cgagacccca ctcagcaccc tcgctgggca 421 cctcaagcct gagggaccca gagccctcag ggaggctggg tgatccagga ccccaggagg 481 cacagacctc gaggtccatc ctggctcaac agagcaagct gtccaagccc agggtgacct 541 tctctgagga gtctgcagtt cctgagagga gctggcgcct caggccatac ctgggctatg 601 actggattgc agggtctctg gacaccagct cttccatcac cagccagcct gaggccttct 661 tctccaagct gcaggagttt cgggaaacca acaaggagga gtgtatctgc agccatcctg 721 aaccccagtt gccaggcctg cgtgagagca gtggcagcgg cgtggaggaa gaccatgaat 781 gcgtgtactg ttaccgtgtc aaccggcgcc tgttcccggt gcctgtggat cccggtaccc 841 cctgccgcct gtgcaggaca ccgcgagacc agcagggccc tgggaccctg gcgcagccag 901 cgcacgtcag ggtgagcatc ccgctgtcga tcctggagcc cccgcaccgg taccacatcc 961 accggcgaaa gagctttgac gcctctgaca cactggccct gccccggcac tgcctgctgg 1021 gctgggacat ttttcctccg aagtctgaga aaagctcagc ccccaggaac ctggacctct 1081 ggtcctctgt ctccgctgag gcccagcacc agaagctgtc cggcaccagc agcccttttc 1141 acccgGCCTC ACCAATGCAG ATGCTGCCCC CGACCCCGAC CTGGTCAGTG CCCCAGGTCC 1201 CTCGGCCCCA CGTCCCACGG CAGAAGCCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1261 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1321 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAATCA 1381 CTTTTCTTAA TGTCTCAACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1441 tgaaagatt