Construct: ORF TRCN0000473170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004609.1_s317c1
- Derived from:
- ccsbBroadEn_12596
- DNA Barcode:
- CGGGGACGGCGCTGCTCCGGTGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNT12 (79695)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473170
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79695 | GALNT12 | polypeptide N-acetylgalacto... | XM_006717287.1 | 95.5% | 95.7% | 1_36del;606C>T;816G>C |
| 2 | human | 79695 | GALNT12 | polypeptide N-acetylgalacto... | XM_024447673.1 | 79.1% | 69.5% | (many diffs) |
| 3 | human | 79695 | GALNT12 | polypeptide N-acetylgalacto... | XM_011519020.1 | 78.6% | 78.8% | 1_219del;789C>T;999G>C |
| 4 | human | 79695 | GALNT12 | polypeptide N-acetylgalacto... | XM_017015133.1 | 54.5% | 54.7% | 1_675del;1245C>T;1455G>C |
| 5 | human | 79695 | GALNT12 | polypeptide N-acetylgalacto... | XM_011519018.3 | 53.5% | 53.6% | 1_705del;1275C>T;1485G>C |
| 6 | human | 79695 | GALNT12 | polypeptide N-acetylgalacto... | NM_024642.5 | 46.7% | 46.8% | 1_927del;1497C>T;1707G>C |
| 7 | mouse | 230145 | Galnt12 | UDP-N-acetyl-alpha-D-galact... | XR_881062.2 | 24.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 882
- ORF length:
- 816
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tggtgggctg tttgctgtga gtaagaaata ttttgaatat ctggggtctt 121 atgatacagg aatggaagtt tggggaggag aaaacctcga attttccttt aggatctggc 181 agtgtggtgg ggttctggaa acacacccat gttcccatgt tggccatgtt ttccccaagc 241 aagctcccta ctcccgcaac aaggctctgg ccaacagtgt tcgtgcagct gaagtatgga 301 tggatgaatt taaagagctc tactaccatc gcaacccccg tgcccgcttg gaaccttttg 361 gggatgtgac agagaggaag cagctccggg acaagctcca gtgtaaagac ttcaagtggt 421 tcttggagac tgtgtatcca gaactgcatg tgcctgagga caggcctggc ttcTTCGGGA 481 TGCTCCAGAA CAAAGGACTA ACAGACTACT GCTTTGACTA TAACCCTCCC GATGAAAACC 541 AGATTGTGGG ACACCAGGTC ATTCTGTACC TCTGTCATGG GATGGGCCAG AATCAGTTTT 601 TCGAGTACAC GTCCCAGAAA GAAATACGCT ATAATACCCA CCAGCCTGAG GGCTGCATTG 661 CTGTGGAAGC AGGAATGGAT ACCCTTATCA TGCATCTCTG CGAAGAAACT GCCCCAGAGA 721 ATCAGAAGTT CATCTTGCAG GAGGATGGAT CTTTATTTCA CGAACAGTCC AAGAAATGTG 781 TCCAGGCTGC GAGGAAGGAG TCGAGTGACA GTTTCGTTCC ACTCTTACGA GACTGCACCA 841 ACTCCGATCA TCAGAAATGG TTCTTCAAAG AGCGCATGTT ATGCCCAACT TTCTTGTACA 901 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 961 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1021 AGGACGACGG GGACGGCGCT GCTCCGGTGA AACGCGTTAA GTCgacaatc aacctctgga 1081 ttacaaaatt tgtgaaagat t