Construct: ORF TRCN0000473180
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009439.1_s317c1
- Derived from:
- ccsbBroadEn_14457
- DNA Barcode:
- AGAATGGATGTTCGATCTTTTATC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R45 (259291)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473180
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259291 | TAS2R45 | taste 2 receptor member 45 | NM_176886.2 | 99.3% | 97.6% | (many diffs) |
| 2 | human | 259289 | TAS2R43 | taste 2 receptor member 43 | XM_003960991.5 | 99.3% | 97.6% | (many diffs) |
| 3 | human | 259289 | TAS2R43 | taste 2 receptor member 43 | NM_176884.2 | 89.6% | 83.1% | (many diffs) |
| 4 | human | 259290 | TAS2R31 | taste 2 receptor member 31 | NM_176885.2 | 89.3% | 81.5% | (many diffs) |
| 5 | human | 259292 | TAS2R46 | taste 2 receptor member 46 | NM_176887.2 | 89.1% | 82.2% | (many diffs) |
| 6 | human | 259293 | TAS2R30 | taste 2 receptor member 30 | NM_001097643.1 | 85.5% | 75.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 966
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gataactttt ctgcccatca tattttccat tctagtagtg gttacatttg 121 ttattggaaa ttttgctaat ggcttcatag cgttggtaaa ttccaccgag tgggtgaaga 181 gacaaaagat ctcctttgct gaccaaattg tcactgctct ggcggtctcc agagttggtt 241 tgctctgggt gttattatta aattggtatt caactgtgtt gaatccagct ttttgtagtg 301 tagaattaag aactactgct tataatatct gggcagtaac cggccatttc agcaactggc 361 ctgctactag cctcagcata ttttatttgc tcaagattgc caatttctcc aaccttattt 421 ttcttcgctt aaagaggaga gttaagagtg tcattctggt gatgctgttg gggcctttgc 481 tatttttggc ttgtcatctt tttgtggtaa acatgaatca gattgtatgg acaaaagaat 541 atgaaggaaa catgacttgg aagatcaaat tgaggcgtgc aatgtaCCTT TCAGATACGA 601 CTGTAACCAT GCTAGCAAAC TTAGTACCCT TTACTGTAAC CCTGATATCT TTTCTGCTGT 661 TAGTCTGTTC TCTGTGTAAA CATCTCAAGA AGATGCACCT CCATGGCAAA GGATCTCAAG 721 ATCCCAGTAC CAAGGTCCAC ATAAAAGTTT TGCAAACTGT GATCTCCTTC CTCTTGTTAT 781 GTGCCATTTA CTTTGTGTCT GTAATAATAT CAGTTTGGAG TTTTAAGAAT CTGGAAAACA 841 AACCTGTCTT CATGTTCTGC CAAGCTATTG GATTCAGCTG TTCTTCAGCC CACCCGTTCA 901 TCCTGATTTG GGGAAACAAG AAGCTAAAGC AGACTTATCT TTCAGTTTTG TGGCAAATGG 961 GTACTAGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 1021 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 1081 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGAATGGATG TTCGATCTTT TATCACGCGT 1141 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt