Construct: ORF TRCN0000473186
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016674.1_s317c1
- Derived from:
- ccsbBroadEn_06138
- DNA Barcode:
- ACTTGTCCAAAGGTGGCTCAATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EDNRB (1910)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473186
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1910 | EDNRB | endothelin receptor type B | NM_000115.5 | 99.8% | 100% | 552T>C;831A>G |
| 2 | human | 1910 | EDNRB | endothelin receptor type B | NM_001122659.3 | 99.8% | 100% | 552T>C;831A>G |
| 3 | human | 1910 | EDNRB | endothelin receptor type B | NM_003991.4 | 93.2% | 89.1% | (many diffs) |
| 4 | human | 1910 | EDNRB | endothelin receptor type B | NM_001201397.1 | 83% | 83% | 1_270del;1101A>G |
| 5 | mouse | 13618 | Ednrb | endothelin receptor type B | NM_001136061.2 | 85.1% | 88.7% | (many diffs) |
| 6 | mouse | 13618 | Ednrb | endothelin receptor type B | NM_001276296.1 | 85.1% | 88.7% | (many diffs) |
| 7 | mouse | 13618 | Ednrb | endothelin receptor type B | NM_007904.4 | 85.1% | 88.7% | (many diffs) |
| 8 | mouse | 13618 | Ednrb | endothelin receptor type B | XM_006518515.2 | 85.1% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1392
- ORF length:
- 1326
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gccgcctcca agtctgtgcg gacgcgccct ggttgcgctg gttcttgcct 121 gcggcctgtc gcggatctgg ggagaggaga gaggcttccc gcctgacagg gccactccgc 181 ttttgcaaac cgcagagata atgacgccac ccactaagac cttatggccc aagggttcca 241 acgccagtct ggcgcggtcg ttggcacctg cggaggtgcc taaaggagac aggacggcag 301 gatctccgcc acgcaccatc tcccctcccc cgtgccaagg acccatcgag atcaaggaga 361 ctttcaaata catcaacacg gttgtgtcct gccttgtgtt cgtgctgggg atcatcggga 421 actccacact tctgagaatt atctacaaga acaagtgcat gcgaaacggt cccaatatct 481 tgatcgccag cttggctctg ggagacctgc tgcacatcgt cattgacatc cctatcaatg 541 tctacaagct gctggcagag gactggccat ttggagctga gatgtgtaag ctggtgcctt 601 tcatacagaa agcctccgtg ggaatcactg tgctgagtct atgtgctctg agtattgaca 661 gatatcgagc tgttgcttct tggagtagaa ttaaaggaat tggggttcca aaatggacag 721 cagtagaaat tgttttgatt tgggtggtct ctgtggttct ggctgtccct gaagccatag 781 gttttgatat aattacgatg gactacaaag gaagttatct gcgaatctgc ttgcttcatc 841 ccgttcagaa gacagctttc atgcagtttt acaagacagc aaaagattgg tggctgttca 901 gtttctattt ctgcttgcca ttggccatca ctgcattttt ttatacacta atgacctgtg 961 aaatgttgag aaagaaaagt ggcatgcaga ttgctttaaa tgatcaccTA AAGCAGAGAC 1021 GGGAAGTGGC CAAAACCGTC TTTTGCCTGG TCCTTGTCTT TGCCCTCTGC TGGCTTCCCC 1081 TTCACCTCAG CAGGATTCTG AAGCTCACTC TTTATAATCA GAATGATCCC AATAGATGTG 1141 AACTTTTGAG CTTTCTGTTG GTATTGGACT ATATTGGTAT CAACATGGCT TCACTGAATT 1201 CCTGCATTAA CCCAATTGCT CTGTATTTGG TGAGCAAAAG ATTCAAAAAC TGCTTTAAGT 1261 CATGCTTATG CTGCTGGTGC CAGTCATTTG AAGAAAAACA GTCCTTGGAG GAAAAGCAGT 1321 CGTGCTTAAA GTTCAAAGCT AATGATCACG GATATGACAA CTTCCGTTCC AGTAATAAAT 1381 ACAGCTCATC TTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1441 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1501 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACT TGTCCAAAGG TGGCTCAATC 1561 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t