Construct: ORF TRCN0000473195
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002928.1_s317c1
- Derived from:
- ccsbBroadEn_15632
- DNA Barcode:
- ACCAAGGGCCACGGTAGCTGATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYRK2 (8445)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473195
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8445 | DYRK2 | dual specificity tyrosine p... | NM_003583.4 | 99.8% | 99.8% | 1272G>T;1569G>C |
| 2 | human | 8445 | DYRK2 | dual specificity tyrosine p... | XM_017020032.1 | 99.8% | 99.8% | 1272G>T;1569G>C |
| 3 | human | 8445 | DYRK2 | dual specificity tyrosine p... | NM_006482.3 | 87.7% | 87.6% | 1_219del;1491G>T;1788G>C |
| 4 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | XM_011243552.2 | 89.9% | 97.7% | (many diffs) |
| 5 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | NM_001014390.2 | 79% | 85.8% | (many diffs) |
| 6 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | XM_006514046.3 | 71.2% | 77.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1650
- ORF length:
- 1584
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgatcacctg catgtcggca gccacgctca cggacagatc caggttcaac 121 agttgtttga ggataacagt aacaagcgga cagtgctcac gacacaacca aatgggctta 181 caacagtggg caaaacgggc ttgccagtgg tgccagagcg gcagctggac agcattcata 241 gacggcaggg gagctccacc tctctaaagt ccatggaagg catggggaag gtgaaagcca 301 cccccatgac acctgaacaa gcaatgaagc aatacatgca aaaactcaca gccttcgaac 361 accatgagat tttcagctac cctgaaatat atttcttggg tctaaatgct aagaagcgcc 421 agggcatgac aggtgggccc aacaatggtg gctatgatga tgaccaggga tcatatgtgc 481 aggtgcccca cgatcacgtg gcttacaggt atgaggtcct caaggtcatt gggaagggga 541 gctttgggca ggtggtcaag gcctacgatc acaaagtcca ccagcacgtg gccctaaaga 601 tggtgcggaa tgagaagcgc ttccaccggc aagcagcgga ggagatccga atcctggaac 661 acctgcggaa gcaggacaag gataacacaa tgaatgtcat ccatatgctg gagaatttca 721 ccttccgcaa ccacatctgc atgacgtttg agctgctgag catgaacctc tatgagctca 781 tcaagaagaa taaattccag ggcttcagtc tgcctttggt tcgcaagttt gcccactcga 841 ttctgcagtg cttggatgct ttgcacaaaa acagaataat tcactgtgac cttaagcccg 901 agaacatttt gttaaagcag cagggtagaa gcggtattaa agtaattgat tttggctcca 961 gttgttacga gcatcagcgt gtctacacgt acatccagtc gcgtttttac cgggctccag 1021 aagtgatcct tggggccagg tatggcatgc ccattgatat gtggagcctg ggctgcattt 1081 tagcagagct cctgacgggt taccccctct tgcctgggga agatgaaggg gaccagctgg 1141 cctgtatgat tgaactgttg ggcatgccct cacagaaact gctggatgca tccaaacgag 1201 ccaaaaattt tgtgagctcc aagggttatc cccgttactg cactgtcacg actctctcag 1261 atggctctgt ggtccTAAAC GGAGGCCGTT CCCGGAGGGG GAAACTGAGG GGCCCACCGG 1321 AGAGCAGAGA GTGGGGTAAC GCGCTGAAGG GGTGTGATGA TCCCCTTTTC CTTGACTTCT 1381 TAAAACAGTG TTTAGAGTGG GATCCTGCAG TGCGCATGAC CCCAGGCCAG GCTTTGCGGC 1441 ACCCCTGGCT GAGGAGGCGG TTGCCAAAGC CTCCCACCGG GGAGAAAACG TCAGTGAAAA 1501 GGATAACTGA GAGCACCGGT GCTATCACAT CTATATCCAA GTTACCTCCA CCTTCTAGCT 1561 CAGCTTCCAA ACTGAGGACT AATTTGGCGC AGATGACAGA TGCCAATGGG AATATTCAGC 1621 AGAGGACAGT GTTCCCAAAA CTTGTTAGCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1681 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1741 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACCAA 1801 GGGCCACGGT AGCTGATTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1861 tgaaagatt