Construct: ORF TRCN0000473198
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003334.1_s317c1
- Derived from:
- ccsbBroadEn_11554
- DNA Barcode:
- TATGGCGAGCGGGCTATAATTAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENTR1 (10807)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473198
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10807 | ENTR1 | endosome associated traffic... | NM_001039708.2 | 95.6% | 91.1% | (many diffs) |
| 2 | human | 10807 | ENTR1 | endosome associated traffic... | XM_005266051.3 | 91.1% | 88.1% | (many diffs) |
| 3 | human | 10807 | ENTR1 | endosome associated traffic... | XM_011518157.2 | 85.8% | 84.4% | (many diffs) |
| 4 | human | 10807 | ENTR1 | endosome associated traffic... | NM_006643.4 | 85.6% | 85.4% | (many diffs) |
| 5 | human | 10807 | ENTR1 | endosome associated traffic... | NM_001039707.2 | 81.1% | 80.9% | (many diffs) |
| 6 | human | 10807 | ENTR1 | endosome associated traffic... | XM_005266050.4 | 79% | 78.8% | (many diffs) |
| 7 | human | 10807 | ENTR1 | endosome associated traffic... | XM_017014218.2 | 72.9% | 72.7% | (many diffs) |
| 8 | human | 10807 | ENTR1 | endosome associated traffic... | XM_017014219.2 | 71% | 70.8% | (many diffs) |
| 9 | human | 10807 | ENTR1 | endosome associated traffic... | XR_002956746.1 | 42.9% | (many diffs) | |
| 10 | human | 10807 | ENTR1 | endosome associated traffic... | XR_001746168.2 | 40.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ctatgtgccc agcccggtgc tggcttccgt gggagacaca gatgacagat 121 ttgaagatct ggaagaggca aatccattct cttttagaga gtttctgaag accaagaacc 181 tcggcctctc gaaagaggat ccggccagca gaatttatgc aaaggaagcc tcgaggcatt 241 ccctgggact tgaccacaac tccccaccct cccaaaccgg cgggtatggc ctggagtatc 301 agcagccatt tttcgaggat ccgacagggg ctggtgacct cctggatggg gaggaggatg 361 aggacaccgg atggagtggg gcctacctgc cgtccgccat cgagcagact caccccgaga 421 gggtccctgc cggcacgtcg ccctgcagca catacctttc ctttttctcc accccgtcgg 481 agctggcagg gcctgagtct ctgccctcgt gggcgttgag tgacactgat tctcgcgtgt 541 ctccggcctc tccggcaggg agtcctagcg cagactttgc ggttcatgga gagtctctgg 601 gagacaggca cctgcggacg ctgcagataa gttacgacgc actgaaagat gaaaattcta 661 agctgagaag aaagctgaat gaggttcaga gcttctctga agctcaaaca gaaatggtga 721 ggacgcttga gcggaagtta gaagcaaaaa tgatcaagga ggaaagcgac taccacgacc 781 tGGAGTCGGT GGTTCAGCAG GTGGAGCAGA ACCTGGAGCT GATGACCAAA CGGGCTGTAA 841 AGGCAGAAAA CCACGTCGTG AAACTAAAAC AGGAAATCAG TTTGCTCCAG GCGCAGGTCT 901 CCAACTTCCA GCGAGAGAAT GAAGCCCTGC GGTGCGGCCA GGGCGCCAGC CTGACCGTGG 961 TGAAGCAGAA CGCCGACGTG GCCCTGCAGA ACCTCCGGGT GGTCATGAAC AGTGCACAGG 1021 CTTCCATCAA GCAACTGGTT TCCGGAGCTG AGACACTGAA TCTTGTTGCC GAAATCCTTA 1081 AATCTATAGA CAGAATTTCT GAAATTAAAG ACGAGGAGGA AGACTCTTGC CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGATATGGCG AGCGGGCTAT AATTAGAACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt