Construct: ORF TRCN0000473199
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015164.1_s317c1
- Derived from:
- ccsbBroadEn_15870
- DNA Barcode:
- AAAGGCCTTTTCGCACGCCTTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HAUS6 (54801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473199
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54801 | HAUS6 | HAUS augmin like complex su... | XM_011517935.2 | 67.4% | 67.1% | 1_510del;551C>T;989G>T |
| 2 | human | 54801 | HAUS6 | HAUS augmin like complex su... | NM_001270890.2 | 38.4% | 38.2% | 1_1698del;1739C>T;2177G>T |
| 3 | human | 54801 | HAUS6 | HAUS augmin like complex su... | NM_017645.5 | 36.9% | 36.8% | 1_1803del;1844C>T;2282G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaaaacaga actaaagaac caattcaaat ggatgttgaa catagagaag 121 tattgccaga atcattacct gtgttgcaca atcaaagaga atttagcatg gctgattttc 181 tcttagaaac cactgtatca gattttggcc agtctcattt gactgaagag aaagttattt 241 cagattgcga gtgtgtgcct cagaaacatg tgctgaccag tcacatagat gaaccaccaa 301 cacaaaatca gtcagatttg ttaaataaga aagtaatttg caagcaagat ttggaatgtt 361 tagccttcac caagctttca gaaactagcc gaatggagac attctcccct gctgtcggca 421 ataggataga tgtgatgggt ggcagtgaag aggagtttat gaaaatattg gaccacttag 481 aagtttcttg taacaaacct tccacaaata aaactatgtt gtggaattct tttcagatat 541 caattggaat tagttctaag agttttaaag ataatgattt tggcatatta cacgaaactc 601 tcccggaaga agttggtcat ctaagtttta atagttccag tagttcagag gccaatttta 661 aactggagcc aaatagtcct atgcatggtg gcactcttcT AGAAGATGTT GTGGGAGGGA 721 GACAGACTAC TCCAGAATCA GACTTTAATT TACAGGCTCT TCGCAGTAGA TACGAGGCTC 781 TGAAGAAATC TCTTTCCAAG AAAAGGGAAG AATCTTACCT CTCGAATTCC CAAACACCCG 841 AAAGACACAA ACCAGAATTG AGCCCTACTC CCCAAAATGT ACAAACAGAT GATACGCTTA 901 ACTTTTTGGA CACCTGTGAT TTGCATACTG AGCATATAAA GCCATCTTTA CGCACGTCCA 961 TCGGTGAAAG AAAACGGTCT CTTTCACCAC TAATTAAGTT TTCTCCAGTG GAACAAAGAT 1021 TGAGAACCAC AATAGCATGT AGTCTTGGAG AACTACCTAA TTTAAAGGAA GAAGACATTT 1081 TGAATAAGAG CCTTGATGCA AAAGAACCAC CGTCTGACTT GACAAGATGC CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGAAAAGGCC TTTTCGCACG CCTTGTCACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt