Construct: ORF TRCN0000473205
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013318.1_s317c1
- Derived from:
- ccsbBroadEn_02897
- DNA Barcode:
- ACTGTATGAGACATTGCCAGGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AAR2 (25980)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473205
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25980 | AAR2 | AAR2 splicing factor | NM_001271874.2 | 100% | 100% | |
| 2 | human | 25980 | AAR2 | AAR2 splicing factor | NM_015511.4 | 100% | 100% | |
| 3 | human | 25980 | AAR2 | AAR2 splicing factor | XM_006723770.3 | 100% | 100% | |
| 4 | human | 25980 | AAR2 | AAR2 splicing factor | XM_011528762.2 | 100% | 100% | |
| 5 | human | 25980 | AAR2 | AAR2 splicing factor | XM_011528763.2 | 100% | 100% | |
| 6 | mouse | 68295 | Aar2 | AAR2 splicing factor homolog | NM_001164818.1 | 87.4% | 90.6% | (many diffs) |
| 7 | mouse | 68295 | Aar2 | AAR2 splicing factor homolog | NM_026661.4 | 87.4% | 90.6% | (many diffs) |
| 8 | mouse | 68295 | Aar2 | AAR2 splicing factor homolog | XM_006500097.3 | 87.4% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1218
- ORF length:
- 1152
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgccgtgcag atggatcctg agctagccaa gcgcctcttc tttgaagggg 121 ccactgtggt catcctgaac atgcccaagg gaacagagtt tgggattgac tataactcct 181 gggaggtcgg gcccaagttc cggggcgtga agatgatccc tccaggcatc cacttcctcc 241 actacagctc tgtggacaag gctaatccga aggaagtagg ccctcgtatg ggtttcttcc 301 ttagcctgca ccagcggggg ctgacagtgc tgcgctggag cacactcagg gaagaggtag 361 acctgtcccc agccccagag tctgaggtgg aggccatgag ggccaacctc caggagctgg 421 accagttcct ggggccttac ccatatgcca ccctgaagaa gtggatctca ctcaccaact 481 tcatcagcga agccacagtg gagaagctac agcccgagaa tcgacagatc tgtgcctttt 541 ccgatgtgct acctgtgctc tccatgaagc acaccaagga ccgcgtgggg cagaatctac 601 cccgctgtgg cattgagtgc aaaagctacc aagagggcct ggcccggcta ccagagatga 661 agcccagagc cgggacagag atccgcttct cagagctgcc cacgcagatg ttcccagagg 721 gtgccacgcc agctgagata accaagcaca gcatggacct gagctatgcc ctggagactg 781 tgctcaacaa gcagttcccc agcagccccc aggatgtgct tggtgaactc cagtttgctt 841 ttgtgtgctt cctgctgggg aatgtgtacg aggcattTGA GCATTGGAAG CGGCTCCTGA 901 ACCTCCTGTG CCGGTCAGAA GCAGCCATGA TGAAGCACCA CACCCTCTAC ATCAACCTCA 961 TCTCCATCCT GTACCACCAG CTTGGTGAGA TCCCCGCTGA CTTCTTCGTA GACATTGTCT 1021 CCCAAGACAA CTTCCTCACC AGCACCTTAC AGGTTTTCTT TTCCTCTGCC TGCAGCATTG 1081 CCGTGGATGC CACCCTGAGA AAGAAAGCTG AAAAGTTCCA AGCTCACCTG ACCAAGAAGT 1141 TCCGGTGGGA CTTTGCTGCG GAACCTGAGG ACTGTGCCCC GGTGGTGGTG GAGCTCCCTG 1201 AGGGCATCGA GATGGGCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACTGTAT GAGACATTGC 1381 CAGGTTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt