Construct: ORF TRCN0000473209
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006197.1_s317c1
- Derived from:
- ccsbBroadEn_08072
- DNA Barcode:
- TTTTTTTACCTGTTGCTACGCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBGCP4 (27229)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473209
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27229 | TUBGCP4 | tubulin gamma complex assoc... | NM_014444.5 | 99.8% | 99.6% | 7C>T;16C>T |
| 2 | human | 27229 | TUBGCP4 | tubulin gamma complex assoc... | NM_001286414.3 | 99.7% | 99.5% | 7C>T;16C>T;1278_1280delAGC |
| 3 | human | 27229 | TUBGCP4 | tubulin gamma complex assoc... | XM_017022078.2 | 94% | 93.8% | 7C>T;16C>T;1731_1732ins117 |
| 4 | human | 27229 | TUBGCP4 | tubulin gamma complex assoc... | XM_011521454.3 | 93.9% | 93.7% | (many diffs) |
| 5 | human | 27229 | TUBGCP4 | tubulin gamma complex assoc... | XM_011521455.2 | 79.4% | 79.4% | 0_1ins408;870_872delAGC |
| 6 | human | 27229 | TUBGCP4 | tubulin gamma complex assoc... | XR_243092.4 | 47.2% | (many diffs) | |
| 7 | human | 27229 | TUBGCP4 | tubulin gamma complex assoc... | XR_001751226.2 | 34.4% | (many diffs) | |
| 8 | mouse | 51885 | Tubgcp4 | tubulin, gamma complex asso... | NM_001290824.1 | 90.5% | 98% | (many diffs) |
| 9 | mouse | 51885 | Tubgcp4 | tubulin, gamma complex asso... | NM_153387.3 | 90.4% | 97.9% | (many diffs) |
| 10 | mouse | 51885 | Tubgcp4 | tubulin, gamma complex asso... | NR_077245.2 | 60.6% | (many diffs) | |
| 11 | mouse | 51885 | Tubgcp4 | tubulin, gamma complex asso... | NR_110985.1 | 60.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2064
- ORF length:
- 1998
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ctacgaactg ttcttggctc tgagcgggta ccctgggtcc attttcacct 121 ggaacaagcg gagtggcctg caggtatcgc aggacttccc tttcctccac cccagtgaga 181 ccagtgtcct gaatcgactc tgccggctcg gcacagacta tattcgcttc actgagttca 241 ttgaacagta cacgggccat gtgcaacagc aggatcacca tccatctcaa cagggccaag 301 gtgggttaca tggaatctac ctgcgggcct tctgcacagg gctggattct gttttgcagc 361 cttatcgcca agcactgctt gatttggaac aagagttcct gggtgatccc catctctcca 421 tatcacatgt caactacttc ctagaccagt tccagcttct ttttccctct gtgatggttg 481 tagtagaaca aattaaaagt caaaagattc atggttgtca aatcctggaa acagtctaca 541 aacacagctg tggggggttg cctcctgttc gaagtgcact ggaaaaaatc ctggccgttt 601 gtcatggggt catgtataaa cagctctcag cctggatgct ccatggactc ctcttggacc 661 agcatgaaga attctttatc aaacaggggc catcttctgg taatgtcagt gcccagccag 721 aagaggacga ggaggatctg ggcattgggg gactgacagg aaaacaactg agagaactgc 781 aggacttgcg cctgattgag gaagagaaca tgctggcacc atctctgaag cagttttccc 841 tacgagtgga gattttgcca tcctacattc cagtgagggt tgctgaaaaa atcctatttg 901 ttggagaatc tgtccagatg tttgagaatc aaaatgtgaa cctgactaga aaaggatcca 961 ttttgaaaaa ccaggaagac acttttgctg cagagctgca ccgtctcaag cagcagccac 1021 tcttcagctt ggtggacttt gaacaggtgg tggatcgcat tcgcagcact gtggctgagc 1081 atctctggaa gttgatggta gaagaatccg atttactggg tcagctgaag atcattaaag 1141 acttttacct tctgggacgt ggagaactgt ttcaggcctt cattgacaca gctcaacaca 1201 tgttgaaaac accacccact gcagtaactg agcatgatgt gaatgtggcc tttcaacagt 1261 cagcacacaa ggtattgcta gatgatgaca accttctccc tctgttgcac ttgacaatcg 1321 agtatcacgg aaaggagcac aaagatgcta ctcaggcaag agaagggcct tctcgggaaa 1381 cttctccccg ggaagcccct gcatctggct gggcagccct aggtctttcc tacaaagtac 1441 agtggccact acatattctc ttcaccccag ctgtcctgga aaagtacaat gttgttttta 1501 agtacttact gagtgtgcgc cgggtgcaag ctgagctgca gcactgctgg gccctacaaa 1561 tgcagcgcaa gcacctcaag tcgaaccaga ctgatgcaat caagtggcgc ctaagaaatc 1621 acatggcatt tttggtggat aatcttcagt actatctcca ggtagatgtg ttggagtctc 1681 agttctccca gctgcttcat cagatcaatt ctacccgaga ctttGAAAGC ATCCGATTGG 1741 CTCATGACCA CTTCCTGAGC AATTTGCTGG CTCAATCCTT TATCCTATTG AAACCTGTGT 1801 TTCACTGCCT GAATGAAATC CTAGATCTCT GTCACAGTTT TTGTTCGCTG GTCAGTCAGA 1861 ACCTAGGCCC ACTGGATGAG CGTGGAGCCG CCCAGCTGAG CATTCTCGTG AAGGGCTTTA 1921 GCCGCCAGTC TTCACTCCTG TTCAAGATTC TCTCCAGTGT TCGGAATCAT CAGATCAACT 1981 CAGATTTGGC TCAACTACTG TTACGACTAG ATTATAACAA ATACTATACC CAGGCTGGTG 2041 GAACTCTGGG CAGTTTCGGG ATGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2101 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2161 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TTTTTTACCT 2221 GTTGCTACGC CGGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2281 att