Construct: ORF TRCN0000473217
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012085.1_s317c1
- Derived from:
- ccsbBroadEn_11080
- DNA Barcode:
- TCAAACATAATTTAGAAGCCTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTPRO (5800)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473217
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5800 | PTPRO | protein tyrosine phosphatas... | XM_017019725.2 | 61.1% | 60.9% | (many diffs) |
| 2 | human | 5800 | PTPRO | protein tyrosine phosphatas... | NM_002848.4 | 50.1% | 50% | (many diffs) |
| 3 | human | 5800 | PTPRO | protein tyrosine phosphatas... | NM_030667.3 | 49% | 48.8% | (many diffs) |
| 4 | human | 5800 | PTPRO | protein tyrosine phosphatas... | XR_931316.3 | 25.9% | (many diffs) | |
| 5 | mouse | 19277 | Ptpro | protein tyrosine phosphatas... | NM_001164401.1 | 41.6% | 43.6% | (many diffs) |
| 6 | mouse | 19277 | Ptpro | protein tyrosine phosphatas... | NM_011216.3 | 40.6% | 42.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1857
- ORF length:
- 1791
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcacctgccc acggggatac acggcgcccg ccgcctcctg cctctgctct 121 ggctctttgt gctgttcaag aatgctacag ctttccatgt aactgtccaa gatgataata 181 acatcgttgt ctcattagaa gcttcagacg tcatcagtcc agcatctgtg tatgttgtga 241 agataactgg tgaatccaaa aattatttct tcgaatttga ggaattcaac agcactttgc 301 ctcctcctgt tattttcaag gccagttatc atggccttta ttatataatc actctggtag 361 tggtaaatgg aaatgtggtg accaagccat ccagatcaat cactgtgtta acaaaacctc 421 tacctgtaac cagtgtttcc atatatgact ataaaccttc tcctgaaaca ggagtcctgt 481 ttgaaataca ttatccagaa aaatataacg ttttcacaag agtgaacatt agctactggg 541 aaggtaaaga cttccggaca atgctatata aagatttctt taagggaaaa acagtattta 601 atcactggct gccaggaatg tgttatagta atatcacctt tcagctggta tctgaggcaa 661 cttttaataa aagtaccctt gttgagtaca gtggtgtcag tcacgaaccc aaacagcaca 721 gaactgcccc ttatccacct caaaatattt ccgttcgtat cgtaaacttg aacaaaaaca 781 actgggaaga acagagtggc aatttcccag aagaatcctt catgagatca caagatacaa 841 taggaaaaga aaaactcttc cattttacag aagaaacccc tgaaattccc tcgggcaaca 901 tttcttccgg ttggcctgat tttaatagca gtgactatga aactacgtct cagccatatt 961 ggtgggacag tgcatctgca gctcctgaaa gtgaagatga atttgtcagc gtacttccca 1021 tggaatacga aaataacagt acactcagtg agacagagaa gtcaacatca ggctctttct 1081 cctttttccc tgtgcaaatg atattgacct ggttaccacc caaaccaccc actgcttttg 1141 atgggttcca tatccatatt gaacgagaag agaactttac tgaatatttg atggtggatg 1201 aagaagcaca tgaatttgtt gcagaactga aggaacctgg gaaatataag ttatctgtga 1261 caacctttag ttcctcagga tcttgtgaaa ctcgaaaaag tcagtcagca aaatcactca 1321 gcttttatat cagtccttca ggagagtgga ttgaagaact gaccgagaag ccgcagcacg 1381 tgagtgtcca cgttttaagc TCAACCACTG CCTTGATGTC CTGGACATCT TCCCAAGAGA 1441 ACTACAACAG CACCATTGTG TCTGTGGTGT CGCTGACCTG CCAGAAACAA AAGGAGAGCC 1501 AGAGGCTTGA AAAGCAGTAC TGCACTCAGG TGAACTCAAG CAAACCTATT ATTGAAAATC 1561 TGGTTCCTGG TGCCCAGTAC CAGGTTGTAA TATACCTAAG GAAAGGCCCT TTGATTGGAC 1621 CACCTTCAGA TCCTGTGACA TTTGCTATTG TTCCCACAGG AATAAAGGAT TTAATGCTCT 1681 ATCCTTTGGG TCCTACGGCC GTGGTTCTGA GCTGGACCAG ACCTTATTTA GGCGTGTTCA 1741 GAAAATACGT GGTTGAAATG TTTTATTTCA ACCCTGCTAC AATGACATCA GAGTGGACCA 1801 CCTACTATGA AATAGCAGCA ACTGTTTCCT TAACTGCATC CGTGGTAATC TTCCCTTACC 1861 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1921 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1981 ATATATCTTG TGGAAAGGAC GATCAAACAT AATTTAGAAG CCTTCAACGC GTTAAGTCga 2041 caatcaacct ctggattaca aaatttgtga aagatt