Construct: ORF TRCN0000473229
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010040.1_s317c1
- Derived from:
- ccsbBroadEn_03757
- DNA Barcode:
- TCCTCGCCTTTACCTCGTTTATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CA10 (56934)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473229
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56934 | CA10 | carbonic anhydrase 10 | NM_001082533.1 | 100% | 100% | |
| 2 | human | 56934 | CA10 | carbonic anhydrase 10 | NM_001082534.1 | 100% | 100% | |
| 3 | human | 56934 | CA10 | carbonic anhydrase 10 | NM_020178.5 | 100% | 100% | |
| 4 | human | 56934 | CA10 | carbonic anhydrase 10 | XM_017024878.2 | 70.4% | 70.4% | 0_1ins291 |
| 5 | mouse | 72605 | Car10 | carbonic anhydrase 10 | NM_028296.3 | 94.5% | 100% | (many diffs) |
| 6 | mouse | 72605 | Car10 | carbonic anhydrase 10 | XM_006534319.3 | 94.5% | 100% | (many diffs) |
| 7 | mouse | 72605 | Car10 | carbonic anhydrase 10 | XM_017314795.1 | 94.5% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1050
- ORF length:
- 984
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aatagtctgg gaggtgcttt ttcttcttca agccaatttc atcgtctgca 121 tatcagctca acagaattca ccaaaaatcc atgaaggctg gtgggcatac aaggaggtgg 181 tccagggaag ctttgttcca gttccttctt tctggggatt ggtgaactca gcttggaatc 241 tttgctctgt ggggaaacgg cagtcgccag tcaacataga gaccagtcac atgatcttcg 301 acccctttct gacacctctt cgcatcaaca cggggggcag gaaggtcagt gggaccatgt 361 acaacactgg aagacacgta tcccttcgcc tggacaagga gcacttggtc aacatatctg 421 gagggcccat gacatacagc caccggctgg aggagatccg actacacttt gggagtgagg 481 acagccaagg gtcggagcac ctcctcaatg gacaggcctt ctctggggag gtgcagctca 541 tccactataa ccatgagcta tatacgaatg tcacagaagc tgcaaagagT CCAAATGGAT 601 TGGTGGTAGT TTCTATATTT ATAAAAGTTT CTGATTCATC AAACCCATTT CTTAATCGAA 661 TGCTCAACAG AGATACTATC ACAAGAATAA CATATAAAAA TGATGCATAT TTACTACAGG 721 GGCTTAATAT AGAGGAACTA TATCCAGAGA CCTCTAGTTT CATCACTTAC GATGGGTCGA 781 TGACTATCCC ACCCTGCTAT GAGACAGCAA GTTGGATCAT AATGAACAAA CCTGTCTATA 841 TAACCAGGAT GCAGATGCAT TCCTTGCGCC TGCTCAGCCA GAACCAGCCA TCTCAGATCT 901 TTCTGAGCAT GAGTGACAAC TTCAGGCCTG TCCAGCCACT CAACAACCGC TGCATCCGCA 961 CCAATATCAA CTTCAGTTTA CAGGGGAAGG ACTGTCCAAA CAACCGAGCC CAGAAGCTTC 1021 AGTATAGAGT AAATGAATGG CTCCTCAAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCCTC 1201 GCCTTTACCT CGTTTATAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1261 tgaaagatt