Construct: ORF TRCN0000473231
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005975.1_s317c1
- Derived from:
- ccsbBroadEn_02656
- DNA Barcode:
- TTTATTATGATGATGGTGAGCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CA5B (11238)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473231
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11238 | CA5B | carbonic anhydrase 5B | NM_007220.4 | 100% | 100% | |
| 2 | human | 114004397 | CA5BP1-CA5B | CA5BP1-CA5B readthrough | NR_160544.1 | 11.7% | 1_764del;1103_1753del;2367_8116del | |
| 3 | mouse | 56078 | Car5b | carbonic anhydrase 5b, mito... | NM_181315.4 | 87.7% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1017
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggtgatgaac agcctgaggg tcattcttca agcctctcca ggcaaattgc 121 tgtggagaaa gttccagatt ccgagattca tgccagcgag gccctgcagc ctctatactt 181 gtacttacaa aacccggaac cgagccttgc atccactctg ggagagcgtg gacctggttc 241 ctgggggcga tcgccagtca cccatcaaca ttcggtggag ggacagtgtt tatgatcccg 301 gcttaaaacc actgaccatc tcttatgacc cagccacctg cctccacgtc tggaataatg 361 ggtactcttt cctcgtggaa tttgaagatt ctacagataa atcagtgatc aagggaggac 421 ccctggaaca caactaccga ttgaagcagt tccattttca ctggggggcc atcgatgcct 481 ggggttctga gcacaccgtg gacagcaaat gcttcccagc agagctgcac ttagtgcatt 541 ggaacgcagt cagatttgaa aactttgagg atgcagcact GGAAGAAAAT GGTTTGGCTG 601 TGATAGGAGT ATTTTTAAAG CTAGGCAAAC ATCATAAGGA GCTACAGAAA TTAGTGGATA 661 CTTTGCCGTC AATTAAGCAT AAGGACGCCC TTGTGGAATT TGGGTCATTT GACCCTTCCT 721 GCCTGATGCC TACCTGCCCA GATTACTGGA CCTACTCAGG GTCTCTGACT ACCCCACCCC 781 TCTCCGAGTC TGTCACCTGG ATCATTAAGA AGCAACCAGT AGAGGTTGAT CATGATCAGC 841 TTGAGCAATT TCGGACCCTG CTTTTCACTT CCGAAGGGGA GAAAGAGAAA AGAATGGTGG 901 ACAACTTCCG CCCCCTTCAG CCACTGATGA ATCGCACTGT TCGTTCATCC TTCCGGCATG 961 ATTATGTGCT GAATGTACAA GCGAAACCCA AGCCGGCCAC CAGCCAAGCA ACCCCCTACC 1021 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1081 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1141 ATATATCTTG TGGAAAGGAC GATTTATTAT GATGATGGTG AGCTTCACGC GTTAAGTCga 1201 caatcaacct ctggattaca aaatttgtga aagatt