Construct: ORF TRCN0000473252
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009175.1_s317c1
- Derived from:
- ccsbBroadEn_01325
- DNA Barcode:
- ATTTACGTGAGGTGCGGCCAACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMD12 (5718)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473252
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5718 | PSMD12 | proteasome 26S subunit, non... | NM_002816.5 | 100% | 100% | |
| 2 | human | 5718 | PSMD12 | proteasome 26S subunit, non... | NM_174871.4 | 95.6% | 95.6% | 108_109ins60 |
| 3 | human | 5718 | PSMD12 | proteasome 26S subunit, non... | XM_024450842.1 | 94% | 94% | 169_255del |
| 4 | human | 5718 | PSMD12 | proteasome 26S subunit, non... | NM_001316341.2 | 87% | 87% | 0_1ins177 |
| 5 | human | 5718 | PSMD12 | proteasome 26S subunit, non... | XM_024450843.1 | 87% | 87% | 0_1ins177 |
| 6 | human | 5718 | PSMD12 | proteasome 26S subunit, non... | XR_001752571.2 | 31% | 1_79del;988_1019del;1480_4409del | |
| 7 | mouse | 66997 | Psmd12 | proteasome (prosome, macrop... | NM_025894.2 | 88.1% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1434
- ORF length:
- 1368
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacggcggc tcggagcggg ctgacgggcg catcgtcaag atggaggtgg 121 actacagcgc cacggtggat cagcgcctac ccgagtgtgc gaagctagcc aaggaaggaa 181 gacttcaaga agtcattgaa acccttctct ctctggaaaa gcagactcgt actgcttccg 241 atatggtatc gacatcccgt atcttagttg cagtagtgaa gatgtgctat gaggctaaag 301 aatgggattt acttaatgaa aatattatgc ttttgtccaa aaggcggagt cagttaaaac 361 aagctgttgc caaaatggtt caacagtgct gtacttatgt tgaggaaatc acagaccttc 421 ctatcaaact tcgattaatt gatactctac gaatggttac cgaaggcaag atttatgttg 481 aaattgagcg tgcgcgactg actaaaacat tagcaactat aaaagaacaa aatggtgatg 541 tgaaagaggc agcctccatt ttacaggagt tacaggtgga aacctacggg tcaatggaaa 601 agaaagagcg agtggaattt attttggagc aaatgaggct ctgcctagct gtgaaggatt 661 acattcgaac acaaatcatc agcaagaaaa ttaacaccaa atttttccag gaagaaaata 721 cagagaaatt aaagttgaag tactataatt taatgattca gctggatcaa catgagggat 781 cctatttgtc tatttgtaag cactacagag caatatatga tactccctgt atacaggcag 841 aaagtgaaaa atggcagcag gctctgaaga gtgttgtact ctatgttatc ctggctcctt 901 ttgacaatga acagTCAGAT TTGGTTCACC GAATAAGTGG TGACAAGAAG TTAGAAGAAA 961 TTCCCAAATA CAAGGATCTT TTAAAGCTTT TTACCACAAT GGAGTTGATG CGTTGGTCCA 1021 CACTTGTTGA GGACTATGGA ATGGAATTAA GAAAAGGTTC CCTTGAGAGT CCTGCAACGG 1081 ATGTTTTTGG TTCTACAGAG GAAGGTGAAA AAAGGTGGAA AGACTTGAAG AACAGAGTTG 1141 TTGAACATAA TATTAGAATA ATGGCCAAGT ATTATACTCG GATAACAATG AAAAGGATGG 1201 CACAGCTTCT GGATCTATCT GTTGATGAGT CCGAAGCCTT TCTCTCAAAT CTAGTAGTTA 1261 ACAAGACCAT CTTTGCTAAA GTAGACAGAT TAGCAGGAAT TATCAACTTC CAGAGACCCA 1321 AGGATCCAAA TAATTTATTA AATGACTGGT CTCAGAAACT GAACTCATTA ATGTCTCTGG 1381 TTAACAAAAC TACGCATCTC ATAGCCAAAG AGGAGATGAT ACATAATCTA CAATACCCAA 1441 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1501 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1561 TATCTTGTGG AAAGGACGAA TTTACGTGAG GTGCGGCCAA CACACGCGTT AAGTCgacaa 1621 tcaacctctg gattacaaaa tttgtgaaag att