Construct: ORF TRCN0000473259
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011236.1_s317c1
- Derived from:
- ccsbBroadEn_02196
- DNA Barcode:
- CGGTGCCCTCCCTCCCTCCGATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL33 (9553)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473259
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9553 | MRPL33 | mitochondrial ribosomal pro... | NM_004891.4 | 100% | 100% | |
| 2 | human | 9553 | MRPL33 | mitochondrial ribosomal pro... | NM_145330.2 | 32.7% | 32% | 41_42ins107;89_162del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 215
- ORF end:
- 410
- ORF length:
- 195
- Sequence:
-
1 TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG 61 CACTTGATGT AATTCTCCTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC 121 AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGCTAGCAT 181 CGATTGATCA ACAAGTTTGT ACAAAAAAGT TGGCATGTTC CTCTCCGCGG TCTTCTTTGC 241 CAAGAGCAAG TCAAAAAACA TTCTGGTGAG AATGGTGAGC GAAGCTGGGA CAGGTTTCTG 301 CTTCAACACC AAGAGAAACC GACTGCGGGA AAAACTGACT CTTTTGCATT ATGATCCAGT 361 TGTGAAACAA AGAGTCCTCT TCGTGGAAAA GAAAAAAATA CGCTCCCTTT ACCCAACTTT 421 CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC 481 TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 541 TTGTGGAAAG GACGACGGTG CCCTCCCTCC CTCCGATAAA CGCGTTAAGT Cgacaatcaa 601 cctctggatt acaaaatttg tgaaagatt