Construct: ORF TRCN0000473263
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000327.1_s317c1
- Derived from:
- ccsbBroadEn_09355
- DNA Barcode:
- ACTTCGACGTCTGGAACCAGTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C1orf158 (93190)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473263
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93190 | C1orf158 | chromosome 1 open reading f... | NM_152290.4 | 100% | 100% | |
| 2 | human | 93190 | C1orf158 | chromosome 1 open reading f... | NM_001330361.2 | 80.4% | 80.4% | 109_110ins114 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 648
- ORF length:
- 582
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tttgactgca gtaaatccac agccactctc tactccgagc tggcagattg 121 agaccaagta ttcaacgaaa gtgctcactg gaaattggat ggaagagagg agaaagttca 181 ccagagacac tgacaagaca ccccaatcca tttacagaaa agaatacatc cccttcccag 241 accacagacc agaccagatc tccaggtggt atgggaagag gaaagttgag gggctacctt 301 acaaacacct gatcacccac caccaggagc ccccacatcg ctaccTGATC AGCACCTATG 361 ACGACCATTA CAACCGGCAT GGTTACAACC CGGGGCTGCC TCCACTCCGC ACTTGGAATG 421 GACAGAAGTT GCTGTGGCTG CCAGAGAAGT CTGACTTTCC CCTTCTTGCT CCCCCTACAA 481 ACTATGGACT CTATGAGCAG CTCAAGCAGA GACAGCTCAC ACCCAAGGCT GGCCTGAAGC 541 AGAGCACTTA TACTTCATCC TACCCCAGAC CACCGTTGTG CGCTATGTCC TGGAGGGAGC 601 ATGCGGTCCC GGTCCCTCCC CATCGCCTGC ATCCTCTCCC ACACTTCTGC CCAACTTTCT 661 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 721 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 781 GTGGAAAGGA CGAACTTCGA CGTCTGGAAC CAGTCTCACG CGTTAAGTCg acaatcaacc 841 tctggattac aaaatttgtg aaagatt