Construct: ORF TRCN0000473270
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016326.1_s317c1
- Derived from:
- ccsbBroadEn_06188
- DNA Barcode:
- GCACAGGTACCACCATTGACAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- F10 (2159)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473270
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2159 | F10 | coagulation factor X | NM_000504.4 | 99.9% | 100% | 792C>T |
| 2 | human | 2159 | F10 | coagulation factor X | NM_001312674.2 | 90.9% | 90.7% | 370_371ins132;660C>T |
| 3 | human | 2159 | F10 | coagulation factor X | NM_001312675.2 | 67.9% | 60% | 792C>T;853_854insAGGTGAGGGT;996_997ins458 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1530
- ORF length:
- 1464
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcgcccactg cacctcgtcc tgctcagtgc ctccctggct ggcctcctgc 121 tgctcgggga aagtctgttc atccgcaggg agcaggccaa caacatcctg gcgagggtca 181 cgagggccaa ttcctttctt gaagagatga agaaaggaca cctcgaaaga gagtgcatgg 241 aagagacctg ctcatacgaa gaggcccgcg aggtctttga ggacagcgac aagacgaatg 301 aattctggaa taaatacaaa gatggcgacc agtgtgagac cagtccttgc cagaaccagg 361 gcaaatgtaa agacggcctc ggggaataca cctgcacctg tttagaagga ttcgaaggca 421 aaaactgtga attattcaca cggaagctct gcagcctgga caacggggac tgtgaccagt 481 tctgccacga ggaacagaac tctgtggtgt gctcctgcgc ccgcgggtac accctggctg 541 acaacggcaa ggcctgcatt cccacagggc cctacccctg tgggaaacag accctggaac 601 gcaggaagag gtcagtggcc caggccacca gcagcagcgg ggaggcccct gacagcatca 661 catggaagcc atatgatgca gccgacctgg accccaccga gaaccccttc gacctgcttg 721 acttcaacca gacgcagcct gagaggggcg acaacaacct caccaggatc gtgggaggcc 781 aggaatgcaa ggacggggag tgtccctggc aggccctgct catcaatgag gaaaacgagg 841 gtttctgtgg tggaactatt ctgagcgagt tctacatcct aacggcagcc cactgtctct 901 accaagccaa gagattcaag gtgagggtag gggaccggaa cacggagcag gaggagggcg 961 gtgaggcggt gcacgaggtg gaggtggtca tcaagcacaa ccggttcaca aaggagacct 1021 atgacttcga catcgccgtg ctccggctca agacccccat caccTTCCGC ATGAACGTGG 1081 CGCCTGCCTG CCTCCCCGAG CGTGACTGGG CCGAGTCCAC GCTGATGACG CAGAAGACGG 1141 GGATTGTGAG CGGCTTCGGG CGCACCCACG AGAAGGGCCG GCAGTCCACC AGGCTCAAGA 1201 TGCTGGAGGT GCCCTACGTG GACCGCAACA GCTGCAAGCT GTCCAGCAGC TTCATCATCA 1261 CCCAGAACAT GTTCTGTGCC GGCTACGACA CCAAGCAGGA GGATGCCTGC CAGGGGGACA 1321 GCGGGGGCCC GCACGTCACC CGCTTCAAGG ACACCTACTT CGTGACAGGC ATCGTCAGCT 1381 GGGGAGAGGG CTGTGCCCGT AAGGGGAAGT ACGGGATCTA CACCAAGGTC ACCGCCTTCC 1441 TCAAGTGGAT CGACAGGTCC ATGAAAACCA GGGGCTTGCC CAAGGCCAAG AGCCATGCCC 1501 CGGAGGTCAT AACGTCCTCT CCATTAAAGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1561 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1621 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCACA 1681 GGTACCACCA TTGACAATAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1741 tgaaagatt