Construct: ORF TRCN0000473277
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008635.1_s317c1
- Derived from:
- ccsbBroadEn_06949
- DNA Barcode:
- AGACCCATACACAAGGTAACGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST3GAL1 (6482)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473277
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6482 | ST3GAL1 | ST3 beta-galactoside alpha-... | NM_003033.4 | 99.9% | 100% | 261C>T |
| 2 | human | 6482 | ST3GAL1 | ST3 beta-galactoside alpha-... | NM_173344.3 | 99.9% | 100% | 261C>T |
| 3 | human | 6482 | ST3GAL1 | ST3 beta-galactoside alpha-... | XM_005251025.5 | 99.9% | 100% | 261C>T |
| 4 | human | 6482 | ST3GAL1 | ST3 beta-galactoside alpha-... | XM_006716617.2 | 99.9% | 100% | 261C>T |
| 5 | human | 6482 | ST3GAL1 | ST3 beta-galactoside alpha-... | XM_011517225.2 | 99.9% | 100% | 261C>T |
| 6 | human | 6482 | ST3GAL1 | ST3 beta-galactoside alpha-... | XM_017013736.2 | 99.9% | 100% | 261C>T |
| 7 | human | 6482 | ST3GAL1 | ST3 beta-galactoside alpha-... | XM_024447233.1 | 99.9% | 100% | 261C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1086
- ORF length:
- 1020
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaccctgcgg aagaggaccc tgaaagtgct caccttcctc gtgctcttca 121 tcttcctcac ctccttcttc ctgaactact cccacaccat ggtggccacc acctggttcc 181 ccaagcagat ggtcctggag ctctccgaga acctgaagag actgatcaag cacaggcctt 241 gcacctgcac ccactgcatc gggcagcgca agctctcggc ctggttcgat gagaggttca 301 accagaccat gcagccgctg ctgactgccc agaacgcgct cttggaggac gacacctacc 361 gatggtggct gaggctccag cgggagaaga agcccaataa cttgaatgac accatcaagg 421 agctgttcag agtggtgcct gggaatgtgg accctatgct ggagaagagg tcggtgggct 481 gccggcgctg cgccgttgtg ggcaactcgg gcaacctgag ggagtcttct tatgggcctg 541 agatagacag tcacgacttt gtcctcagga tgaacaaggc gcccacggca gggtttgaag 601 ctgatgttgg gaccaagacc acccaccatc tggtgtaccc TGAGAGCTTC CGGGAGCTGG 661 GAGATAATGT CAGCATGATC CTGGTGCCCT TCAAGACCAT CGACTTGGAG TGGGTGGTGA 721 GCGCCATCAC CACGGGCACC ATTTCCCACA CCTACATCCC GGTTCCTGCA AAGATCAGAG 781 TGAAACAGGA TAAGATCCTG ATCTACCACC CAGCCTTCAT CAAGTATGTC TTTGACAACT 841 GGCTGCAAGG GCACGGGCGA TACCCATCTA CCGGCATCCT CTCGGTCATC TTCTCAATGC 901 ATGTCTGCGA TGAGGTGGAC TTGTACGGCT TCGGGGCAGA CAGCAAAGGG AACTGGCACC 961 ACTACTGGGA GAACAACCCA TCCGCGGGGG CTTTTCGCAA GACGGGGGTG CACGATGCAG 1021 ACTTTGAGTC TAACGTGACG GCCACCTTGG CCTCCATCAA TAAAATCCGG ATCTTCAAGG 1081 GGAGATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGACCCATA CACAAGGTAA CGCGAACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt