Construct: ORF TRCN0000473282
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004669.1_s317c1
- Derived from:
- ccsbBroadEn_02612
- DNA Barcode:
- AACTCTATAGGTTCATAGCCAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUSP14 (11072)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473282
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11072 | DUSP14 | dual specificity phosphatas... | NM_007026.4 | 100% | 100% | |
| 2 | human | 11072 | DUSP14 | dual specificity phosphatas... | XM_005256977.3 | 100% | 100% | |
| 3 | human | 11072 | DUSP14 | dual specificity phosphatas... | XM_011524234.1 | 100% | 100% | |
| 4 | mouse | 56405 | Dusp14 | dual specificity phosphatas... | NM_019819.3 | 90.7% | 93.9% | (many diffs) |
| 5 | mouse | 56405 | Dusp14 | dual specificity phosphatas... | XM_006533796.4 | 90.7% | 93.9% | (many diffs) |
| 6 | mouse | 56405 | Dusp14 | dual specificity phosphatas... | XM_006533797.4 | 90.7% | 93.9% | (many diffs) |
| 7 | mouse | 56405 | Dusp14 | dual specificity phosphatas... | XM_006533798.4 | 90.7% | 93.9% | (many diffs) |
| 8 | mouse | 56405 | Dusp14 | dual specificity phosphatas... | XM_006533799.4 | 90.7% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 660
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ctccagaggt cacagcacgc taccaaggac tctcatggcc cctcggatga 121 tttccgaggg agacatagga ggcattgctc aaatcacctc ctctctattc ctgggcagag 181 gcagtgtggc ctccaatcgg cacctcctcc aggctcgtgg catcaccTGC ATTGTTAATG 241 CTACCATTGA GATCCCTAAT TTCAACTGGC CCCAATTTGA GTATGTTAAA GTGCCTCTGG 301 CTGACATGCC GCATGCCCCC ATTGGACTGT ACTTTGACAC CGTGGCTGAC AAGATCCACA 361 GTGTGAGCAG GAAGCACGGG GCCACCTTGG TGCACTGTGC TGCAGGGGTG AGCCGCTCAG 421 CCACGCTGTG TATCGCGTAC CTGATGAAAT TCCACAACGT GTGCCTGCTG GAGGCGTACA 481 ACTGGGTGAA AGCCCGGCGA CCTGTCATCA GGCCCAACGT AGGCTTCTGG AGGCAACTGA 541 TAGACTACGA GCGCCAGCTC TTTGGGAAGT CGACAGTTAA AATGGTACAG ACACCTTATG 601 GCATAGTTCC CGACGTCTAT GAGAAGGAGT CCCGACACCT GATGCCTTAC TGGGGGATTT 661 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 721 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 781 TTTATATATC TTGTGGAAAG GACGAAACTC TATAGGTTCA TAGCCAAATA CGCGTTAAGT 841 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt