Construct: ORF TRCN0000473284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015781.1_s317c1
- Derived from:
- ccsbBroadEn_00480
- DNA Barcode:
- CCCACAGCTCCTCCTCATCCGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EEF1A1 (1915)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473284
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1915 | EEF1A1 | eukaryotic translation elon... | NM_001402.6 | 100% | 100% | |
| 2 | human | 1917 | EEF1A2 | eukaryotic translation elon... | NM_001958.5 | 77.8% | 92.2% | (many diffs) |
| 3 | human | 106635683 | SNORD141A | small nucleolar RNA, C/D bo... | NR_132980.1 | 7.5% | 0_1ins944;105_106ins337 | |
| 4 | human | 106635684 | SNORD141B | small nucleolar RNA, C/D bo... | NR_132981.1 | 7.5% | 0_1ins944;105_106ins337 | |
| 5 | mouse | 13627 | Eef1a1 | eukaryotic translation elon... | NM_010106.2 | 91.8% | 99.7% | (many diffs) |
| 6 | mouse | 13628 | Eef1a2 | eukaryotic translation elon... | NM_007906.2 | 79.4% | 92.2% | (many diffs) |
| 7 | mouse | 625054 | Gm6548 | predicted gene 6548; eukary... | NR_003363.1 | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1452
- ORF length:
- 1386
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aaaggaaaag actcatatca acattgtcgt cattggacac gtagattcgg 121 gcaagtccac cactactggc catctgatct ataaatgcgg tggcatcgac aaaagaacca 181 ttgaaaaatt tgagaaggag gctgctgaga tgggaaaggg ctccttcaag tatgcctggg 241 tcttggataa actgaaagct gagcgtgaac gtggtatcac cattgatatc tccttgtgga 301 aatttgagac cagcaagtac tatgtgacta tcattgatgc cccaggacac agagacttta 361 tcaaaaacat gattacaggg acatctcagg ctgactgtgc tgtcctgatt gttgctgctg 421 gtgttggtga atttgaagct ggtatctcca agaatgggca gacccgagag catgcccttc 481 tggcttacac actgggtgtg aaacaactaa ttgtcggtgt taacaaaatg gattccactg 541 agccacccta cagccagaag agatatgagg aaattgttaa ggaagtcagc acttacatta 601 agaaaattgg ctacaacccc gacacagtag catttgtgcc aatttctggt tggaatggtg 661 acaacatgct ggagccaagt gctaacatgc cttggttcaa gggatggaaa gtcacccgta 721 aggatggcaa tgccagtgga accacgctgc ttgaggctct ggactgcatc ctaccaccaa 781 ctcgtccaac tgacaagccc ttgcgcctgc ctctccagga tgtctacaaa attggtggta 841 ttggtactgt tcctgttggc cgagtggaga ctggtgttct caaacccggt atggtggTCA 901 CCTTTGCTCC AGTCAACGTT ACAACGGAAG TAAAATCTGT CGAAATGCAC CATGAAGCTT 961 TGAGTGAAGC TCTTCCTGGG GACAATGTGG GCTTCAATGT CAAGAATGTG TCTGTCAAGG 1021 ATGTTCGTCG TGGCAACGTT GCTGGTGACA GCAAAAATGA CCCACCAATG GAAGCAGCTG 1081 GCTTCACTGC TCAGGTGATT ATCCTGAACC ATCCAGGCCA AATAAGCGCC GGCTATGCCC 1141 CTGTATTGGA TTGCCACACG GCTCACATTG CATGCAAGTT TGCTGAGCTG AAGGAAAAGA 1201 TTGATCGCCG TTCTGGTAAA AAGCTGGAAG ATGGCCCTAA ATTCTTGAAG TCTGGTGATG 1261 CTGCCATTGT TGATATGGTT CCTGGCAAGC CCATGTGTGT TGAGAGCTTC TCAGACTATC 1321 CACCTTTGGG TCGCTTTGCT GTTCGTGATA TGAGACAGAC AGTTGCGGTG GGTGTCATCA 1381 AAGCAGTGGA CAAGAAGGCT GCTGGAGCTG GCAAGGTCAC CAAGTCTGCC CAGAAAGCTC 1441 AGAAGGCTAA ATGCGCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1501 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1561 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCC ACAGCTCCTC CTCATCCGGA 1621 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t