Construct: ORF TRCN0000473299
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007478.1_s317c1
- Derived from:
- ccsbBroadEn_14245
- DNA Barcode:
- ACGCCATTTCACGTCCTTGACCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBRS (64319)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473299
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 64319 | FBRS | fibrosin | XM_011545919.3 | 35.3% | 35.3% | (many diffs) |
2 | human | 64319 | FBRS | fibrosin | XM_011545916.3 | 34.9% | 34.9% | (many diffs) |
3 | human | 64319 | FBRS | fibrosin | XM_011545917.3 | 34.9% | 34.9% | (many diffs) |
4 | human | 64319 | FBRS | fibrosin | NM_001105079.3 | 34.5% | 34.5% | (many diffs) |
5 | mouse | 14123 | Fbrs | fibrosin | NM_010183.1 | 65.4% | 68.8% | (many diffs) |
6 | mouse | 14123 | Fbrs | fibrosin | XM_006507348.3 | 31.7% | 33.2% | (many diffs) |
7 | mouse | 14123 | Fbrs | fibrosin | XM_006507347.3 | 31.2% | 32.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1098
- ORF length:
- 1032
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cgtgcgtgtg gcttacatga tcctgagaca ccaggagaaa atgaagggtg 121 actcccacaa gcttgacttt cggaatgacc tcctgccctg ccttccgggg ccctatgggg 181 ccctgccccc tgggcaggag ctctcccacc cggcctccct cttcactgcg actggtgccg 241 tccacgctgc agccaaccct ttcacggcag ctcccggggc ccacggaccc ttcctgagcc 301 ccagcaccca cattgatccc tttgggcgtc ccacaagctt cgcctctttg gctgccctct 361 ccaacggggc ctttggaggc ctgggcagcc ccacattcaa ctccggcgcc gtctttgccc 421 agaaagaaag cccaggggcc ccaccggcct tcgcctcccc accggaccca tggggccgcc 481 tgcaccgcag tcctctgacc tttcctgcct gggtccggcc ccctgaggcc gcccggactc 541 caggctcaga caaggagcgg cctgtggagc ggagggagcc ctccatcacc aaggaggaga 601 aggacaggga cctccccttc tcacggcccc agctccgagt ttctcctgct actcccaagg 661 cccgggctgg tgaggagggg cctcggccaa ccaaggaatc tgtgcggGTA AAGGAAGAGC 721 GGAAGGAGGA GGCTGCCGCC GCCGCTGCCG CTGCTGCTGC CGCCGCCGCT GCCGCCGCCG 781 CAGCAGCCAC TGGGCCCCAG GGCCTTCACC TGCTGTTTGA GAGGCCCCGG CCGCCCCCGT 841 TTCTGGGCCC TAGCCCACCA GATCGCTGTG CTGGCTTCCT GGAGCCAACC TGGTTGGCAG 901 CACCCCCACG CCTGGCAAGG CCACCCCGCT TCTATGAGGC GGGTGAGGAG CTAACTGGAC 961 CCGGGGCCGT GGCCGCTGCC CGCCTCTACG GTCTGGAACC TGCTCACCCC TTGCTCTACA 1021 GCCGCTTGGC TCCTCCACCA CCACCTGCTG CGGCCCCGGG AACCCCTCAC CTTCTCCCTG 1081 CACTCATGAA ACCCCAATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1141 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1201 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACGCCAT TTCACGTCCT 1261 TGACCCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt