Construct: ORF TRCN0000473312
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013670.1_s317c1
- Derived from:
- ccsbBroadEn_09365
- DNA Barcode:
- AAATACCGCAATACTATCGAGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYAP1 (94056)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473312
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 94056 | SYAP1 | synapse associated protein 1 | NM_032796.4 | 99.9% | 99.7% | 77C>T |
| 2 | human | 94056 | SYAP1 | synapse associated protein 1 | NR_033181.2 | 16.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1122
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccggggcttg agcagttggt tgggcttgca gcagccggtg gcaggcggtg 121 ggcagcccaa tggagatgct ctacccgagc agccgtccga gacggtggct gagtctgcgg 181 aggaggagct gcagcaagcg ggagaccagg agctcctcca ccaggccaaa gacttcggca 241 actatttatt taactttgca tctgctgcca caaaaaagat aactgaatca gttgctgaaa 301 cagcacaaac aataaagaaa tccgtagaag aaggaaaaat agatggcatc attgacaaga 361 caattatagg agattttcag aaggaacaga aaaaatttgt tgaagagcaa catacaaaga 421 agtcagaagc agctgtgccc ccatgggttg acactaacga tgaagaaaca attcaacaac 481 aaattttggc cttatcagct gacaagagga atttccttcg tgaccctccg gctggcgtgc 541 aatttaattt cgactttgat cagatgtacc ccgtggccct ggtcatgctc caggaggatg 601 agctgctaag caagatgaga tttgccctcg ttcctaaact tgtgaaggaa gaagtgttct 661 ggaggaacta cttttaccgc gtctccctGA TTAAGCAGTC AGCCCAGCTC ACGGCCCTGG 721 CTGCCCAACA GCAGGCCGCA GGGAAGGAGG AGAAGAGCAA TGGCAGAGAG CAAGATTTGC 781 CGCTGGCAGA GGCAGTACGG CCCAAAACGC CACCCGTTGT AATCAAATCT CAGCTTAAAA 841 CTCAAGAGGA TGAGGAAGAA ATTTCTACTA GCCCAGGTGT TTCTGAGTTT GTCAGTGATG 901 CCTTCGATGC CTGTAACCTA AATCAGGAAG ATCTAAGGAA AGAAATGGAG CAACTAGTGC 961 TTGACAAAAA GCAAGAGGAG ACAGCCGTAC TGGAAGAGGA TTCTGCAGAT TGGGAAAAAG 1021 AACTGCAGCA GGAACTTCAA GAATATGAAG TGGTGACAGA ATCTGAAAAA CGAGATGAAA 1081 ACTGGGATAA GGAAATAGAG AAAATGCTTC AAGAGGAAAA TTACCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGAAAA TACCGCAATA CTATCGAGCG CACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t