Construct: ORF TRCN0000473313
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014700.1_s317c1
- Derived from:
- ccsbBroadEn_07951
- DNA Barcode:
- TGCGGCCATTCGTGTAACCGGAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YIPF3 (25844)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473313
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25844 | YIPF3 | Yip1 domain family member 3 | NM_015388.4 | 99.9% | 100% | 15G>T |
| 2 | mouse | 28064 | Yipf3 | Yip1 domain family, member 3 | NM_145353.2 | 88.7% | 96.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aactacagct gcgccggcgg gcggcgcccg aaatggagct ggcccggaat 121 ggggagggtt cgaagaaaac atccagggcg gaggctcagc tgtgattgac atggagaaca 181 tggatgatac ctcaggctct agcttcgagg atatgggtga gctgcatcag cgcctgcgcg 241 aggaagaagt agacgctgat gcagctgatg cagctgctgc tgaagaggag gatggagagt 301 tcctgggcat gaagggcttt aagggacagc tgagccggca ggtggcagat cagatgtggc 361 aggctgggaa aagacaagcc tccagggcct tcagcttgta cgccaacatc gacatcctca 421 gaccctactt tgatgtggag cctgctcagg tgcgaagcag gctcctggag tccatgatcc 481 ctatcaagat ggtcaacttc ccccagaaaa ttgcaggtga actctatgga cctctcatgc 541 tggtcttcac tctggttgct atcctactcc atgggatgaa gacgtctgac actattatcc 601 gggagggcac cctgatgggc acagccattg gcacctgctt cggctactgg ctgggagtct 661 catccttcat ttacttcctt gcctacctgt gcaacgccca gatcaccatg ctgcagatgt 721 tggcactgct gggctatggc ctctttgggc attgcattgt cctgttcatc acctataata 781 tccacctcca cgccctcttc taccTCTTCT GGCTGTTGGT GGGTGGACTG TCCACACTGC 841 GCATGGTAGC AGTGTTGGTG TCTCGGACCG TGGGCCCCAC ACAGCGGCTG CTCCTCTGTG 901 GCACCCTGGC TGCCCTACAC ATGCTCTTCC TGCTCTATCT GCATTTTGCC TACCACAAAG 961 TGGTAGAGGG GATCCTGGAC ACACTGGAGG GCCCCAACAT CCCGCCCATC CAGAGGGTCC 1021 CCAGAGACAT CCCTGCCATG CTCCCTGCTG CTCGGCTTCC CACCACCGTC CTCAACGCCA 1081 CAGCCAAAGC TGTTGCGGTG ACCCTGCAGT CACACTGCCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 ATGCGGCCAT TCGTGTAACC GGAGAACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt