Construct: ORF TRCN0000473327
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016586.1_s317c1
- Derived from:
- ccsbBroadEn_02019
- DNA Barcode:
- TATCATGGTATTTCGAAGTTTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL18RAP (8807)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473327
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8807 | IL18RAP | interleukin 18 receptor acc... | NM_003853.3 | 100% | 100% | |
| 2 | human | 8807 | IL18RAP | interleukin 18 receptor acc... | XM_024453197.1 | 100% | 100% | |
| 3 | human | 8807 | IL18RAP | interleukin 18 receptor acc... | XM_024453198.1 | 100% | 100% | |
| 4 | human | 8807 | IL18RAP | interleukin 18 receptor acc... | XM_024453199.1 | 100% | 100% | |
| 5 | human | 8807 | IL18RAP | interleukin 18 receptor acc... | XM_024453200.1 | 100% | 100% | |
| 6 | human | 8807 | IL18RAP | interleukin 18 receptor acc... | XM_024453201.1 | 100% | 100% | |
| 7 | human | 8807 | IL18RAP | interleukin 18 receptor acc... | XM_011512087.2 | 76.2% | 76.2% | 0_1ins426 |
| 8 | human | 8807 | IL18RAP | interleukin 18 receptor acc... | XM_011512088.2 | 76.2% | 76.2% | 0_1ins426 |
| 9 | human | 8807 | IL18RAP | interleukin 18 receptor acc... | XR_001739011.2 | 57% | (many diffs) | |
| 10 | human | 8807 | IL18RAP | interleukin 18 receptor acc... | XM_017005173.1 | 51.3% | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1866
- ORF length:
- 1797
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctctgtttg ggctggatat ttctttggct tgttgcagga gagcgaatta 121 aaggatttaa tatttcaggt tgttccacaa aaaaactcct ttggacatat tctacaagga 181 gtgaagagga atttgtctta ttttgtgatt taccagagcc acagaaatca catttctgcc 241 acagaaatcg actctcacca aaacaagtcc ctgagcacct gcccttcatg ggtagtaacg 301 acctatctga tgtccaatgg taccaacaac cttcgaatgg agatccatta gaggacatta 361 ggaaaagcta tcctcacatc attcaggaca aatgtaccct tcactttttg accccagggg 421 tgaataattc tgggtcatat atttgtagac ccaagatgat taagagcccc tatgatgtag 481 cctgttgtgt caagatgatt ttagaagtta agccccagac aaatgcatcc tgtgagtatt 541 ccgcatcaca taagcaagac ctacttcttg ggagcactgg ctctatttct tgccccagtc 601 tcagctgcca aagtgatgca caaagtccag cggtaacctg gtacaagaat ggaaaactcc 661 tctctgtgga aaggagcaac cgaatcgtag tggatgaagt ttatgactat caccagggca 721 catatgtatg tgattacact cagtcggata ctgtgagttc gtggacagtc agagctgttg 781 ttcaagtgag aaccattgtg ggagacacta aactcaaacc agatattctg gatcctgtcg 841 aggacacact ggaagtagaa cttggaaagc ctttaactat tagctgcaaa gcacgatttg 901 gctttgaaag ggtctttaac cctgtcataa aatggtacat caaagattct gacctagagt 961 gggaagtctc agtacctgag gcgaaaagta ttaaatccac tttaaaggat gaaatcattg 1021 agcgtaatat catcttggaa aaagtcactc agcgtgatct tcgcaggaag tttgtttgct 1081 ttgtccagaa ctccattgga aacacaaccc agtccgtcca actgaaagaa aagagaggag 1141 tggtgctcct gtacatcctg cttggcacca tcgggaccct ggtggccgtg ctggcggcga 1201 gtgccctcct ctacaggcac tggattgaaa tagtgctgct gtaccggacc taccagagca 1261 aggatcagac gcttggggat aaaaaggatt ttgatgcttt cgtatcctat gcaaaatgga 1321 gctcttttcc aagtgaggcc acttcatctc tgagtgaaga acacttggcc ctgagcctat 1381 ttccTGATGT TTTAGAAAAC AAATATGGAT ATAGCCTGTG TTTGCTTGAA AGAGATGTGG 1441 CTCCAGGAGG AGTGTATGCA GAAGACATTG TGAGCATTAT TAAGAGAAGC AGAAGAGGAA 1501 TATTTATCTT GAGCCCCAAC TATGTCAATG GACCCAGTAT CTTTGAACTA CAAGCAGCAG 1561 TGAATCTTGC CTTGGATGAT CAAACACTGA AACTCATTTT AATTAAGTTC TGTTACTTCC 1621 AAGAGCCAGA GTCTCTACCT CATCTCGTGA AAAAAGCTCT CAGGGTTTTG CCCACAGTTA 1681 CTTGGAGAGG CTTAAAATCA GTTCCTCCCA ATTCTAGGTT CTGGGCCAAA ATGCGCTACC 1741 ACATGCCTGT GAAAAACTCT CAGGGATTCA CGTGGAACCA GCTCAGAATT ACCTCTAGGA 1801 TTTTTCAGTG GAAAGGACTC AGTAGAACAG AAACCACTGG GAGGAGCTCC CAGCCTAAGG 1861 AATGGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1921 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1981 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATATCATGGT ATTTCGAAGT TTTAGACGCG 2041 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt