Construct: ORF TRCN0000473351
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011961.1_s317c1
- Derived from:
- ccsbBroadEn_02810
- DNA Barcode:
- TCCTCCGCCATTATTTTCTCTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHLDA3 (23612)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473351
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23612 | PHLDA3 | pleckstrin homology like do... | NM_012396.5 | 100% | 100% | |
| 2 | human | 23612 | PHLDA3 | pleckstrin homology like do... | NR_073080.1 | 23.4% | 1_385del;767_1625del | |
| 3 | mouse | 27280 | Phlda3 | pleckstrin homology like do... | NM_013750.2 | 93.1% | 98.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 447
- ORF length:
- 381
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatGAC GGCGGCGGCG ACGGCTACCG TGCTCAAGGA GGGCGTGCTG GAGAAGCGCA 121 GCGGCGGGCT GCTGCAGCTG TGGAAGCGGA AGCGCTGCGT CCTCACCGAA CGCGGGCTGC 181 AGCTCTTCGA GGCCAAGGGC ACGGGCGGCC GGCCCAAGGA GCTCAGCTTC GCCCGCATCA 241 AGGCCGTGGA GTGCGTGGAG AGCACCGGGC GCCACATCTA CTTCACGCTG GTGACCGAAG 301 GGGGCGGCGA GATCGACTTC CGCTGCCCCC TGGAAGATCC CGGCTGGAAC GCCCAGATCA 361 CCCTAGGCCT GGTCAAGTTC AAGAACCAGC AGGCCATCCA GACAGTGCGG GCCCGGCAGA 421 GCCTCGGGAC CGGGACCCTC GTGTCCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCCTCCGC 601 CATTATTTTC TCTTTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt