Construct: ORF TRCN0000473362
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004295.1_s317c1
- Derived from:
- ccsbBroadEn_05862
- DNA Barcode:
- TATATTACTACTTTTGGAAGTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATF1 (466)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473362
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 466 | ATF1 | activating transcription fa... | NM_005171.5 | 99.7% | 99.6% | 5A>N;327C>T |
| 2 | human | 466 | ATF1 | activating transcription fa... | XM_011538386.2 | 99.7% | 99.6% | 5A>N;327C>T |
| 3 | human | 466 | ATF1 | activating transcription fa... | XM_011538387.2 | 99.7% | 99.6% | 5A>N;327C>T |
| 4 | human | 466 | ATF1 | activating transcription fa... | XM_017019332.1 | 99.7% | 99.6% | 5A>N;327C>T |
| 5 | human | 466 | ATF1 | activating transcription fa... | XM_017019333.1 | 99.7% | 99.6% | 5A>N;327C>T |
| 6 | human | 466 | ATF1 | activating transcription fa... | XM_017019334.1 | 99.7% | 99.6% | 5A>N;327C>T |
| 7 | human | 466 | ATF1 | activating transcription fa... | XM_017019331.1 | 96.8% | 96.7% | 1_24del;29A>N;351C>T |
| 8 | human | 466 | ATF1 | activating transcription fa... | XM_011538388.2 | 65.1% | 65.3% | 0_1ins282;45C>T |
| 9 | human | 466 | ATF1 | activating transcription fa... | XM_017019335.1 | 65.1% | 65.3% | 0_1ins282;45C>T |
| 10 | human | 466 | ATF1 | activating transcription fa... | XM_017019336.1 | 65.1% | 65.3% | 0_1ins282;45C>T |
| 11 | mouse | 11908 | Atf1 | activating transcription fa... | NM_007497.3 | 85.8% | 90.7% | (many diffs) |
| 12 | mouse | 11908 | Atf1 | activating transcription fa... | XM_006520351.3 | 85.8% | 90.7% | (many diffs) |
| 13 | mouse | 11908 | Atf1 | activating transcription fa... | XM_006520352.3 | 85.8% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 879
- ORF length:
- 813
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggn agattcccac aagagtacca cgtcagagac agcacctcaa cctggttcag 121 cagttcaggg agctcacatt tctcatattg ctcaacaggt atcatcttta tcagaaagtg 181 aggagtccca ggactcatcc gacagcatag gctcctcaca gaaagcccac gggatcctag 241 cacggcgccc atcttacaga aaaattttga aagacttatc ttctgaagat acacggggca 301 gaaaaggaga cggagaaaat tctggagttt ctgctgctgt cacttctatg tctgttccaa 361 ctcccatcta tcagactagc agcggacagt atattgccat tgccccaaat ggagccttac 421 agttggcaag tccaggcaca gatggagtac agggacttca gacattaacc atgacaaatt 481 caggcagtac tcagcaaggt acaactattc ttcagtatgc acagacctct gatggacagc 541 agatacttgt gcccagcaat caggtggtcg tacaaactgc atcaggagat atgcaaacat 601 atcagatccg aactacacct tcagctactt ctcTGCCACA AACTGTGGTG ATGACATCTC 661 CTGTGACTCT CACCTCTCAG ACAACTAAGA CAGATGACCC CCAATTGAAA AGAGAAATAA 721 GGTTAATGAA AAACAGAGAA GCTGCTCGAG AATGTCGCAG AAAGAAGAAA GAATATGTGA 781 AATGCCTGGA AAACCGAGTT GCAGTCCTGG AAAATCAAAA TAAAACTCTA ATAGAAGAGT 841 TAAAAACTTT GAAGGATCTT TATTCCAATA AAAGTGTTTG CCCAACTTTC TTGTACAAAG 901 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 961 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1021 ACGATATATT ACTACTTTTG GAAGTGGCAC GCGTTAAGTC gacaatcaac ctctggatta 1081 caaaatttgt gaaagatt