Construct: ORF TRCN0000473367
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018447.1_s317c1
- Derived from:
- ccsbBroadEn_00595
- DNA Barcode:
- CAATGTGATTCGAGGCCACTGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NR5A1 (2516)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473367
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2516 | NR5A1 | nuclear receptor subfamily ... | NM_004959.5 | 100% | 100% | |
| 2 | mouse | 26423 | Nr5a1 | nuclear receptor subfamily ... | NM_001316687.1 | 88.3% | 93.5% | (many diffs) |
| 3 | mouse | 26423 | Nr5a1 | nuclear receptor subfamily ... | NM_139051.3 | 88.3% | 93.5% | (many diffs) |
| 4 | mouse | 26423 | Nr5a1 | nuclear receptor subfamily ... | XM_006498056.3 | 81.9% | 86.7% | (many diffs) |
| 5 | mouse | 26423 | Nr5a1 | nuclear receptor subfamily ... | XM_006498057.3 | 81.9% | 86.7% | (many diffs) |
| 6 | mouse | 26423 | Nr5a1 | nuclear receptor subfamily ... | XM_006498058.3 | 81.9% | 86.7% | (many diffs) |
| 7 | mouse | 26423 | Nr5a1 | nuclear receptor subfamily ... | XM_006498059.3 | 81.9% | 86.7% | (many diffs) |
| 8 | mouse | 26423 | Nr5a1 | nuclear receptor subfamily ... | XM_006498060.3 | 81.9% | 86.7% | (many diffs) |
| 9 | mouse | 26423 | Nr5a1 | nuclear receptor subfamily ... | XM_017318656.1 | 61.6% | 59.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1449
- ORF length:
- 1383
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctattcgtac gacgaggacc tggacgagct gtgccccgtg tgcggggaca 121 aggtgtccgg ctaccactac ggactgctca cgtgtgagag ctgcaagggc ttcttcaagc 181 gcacggtgca gaacaacaag cactacacgt gcaccgagag ccagagctgc aagatcgaca 241 agacgcagcg caagcgctgt cccttctgcc gcttccagaa atgcctgacg gtggggatgc 301 gcctggaagc cgtgcgcgct gaccgtatga ggggtggccg gaacaagttt gggccgatgt 361 acaagcggga ccgggccctg aaacagcaga agaaggcaca gattcgggcc aatggcttca 421 agctggagac agggcccccg atgggggtgc ccccgccgcc ccctcccgca ccggactacg 481 tgctgcctcc cagcctgcat gggcctgagc ccaagggcct ggccgccggt ccacctgctg 541 ggccactggg cgactttggg gccccagcac tgcccatggc cgtgcccggt gcccacgggc 601 cactggctgg ctacctctac cctgcctttc ctggccgtgc catcaagtct gagtacccgg 661 agccttatgc cagcccccca cagcctgggc tgccgtacgg ctacccagag cccttctctg 721 gagggcccaa cgtgcctgag ctcatcctgc agctgctgca gctggagccg gatgaggacc 781 aggtgcgggc ccgcatcttg ggctgcctgc aggagcccac caaaagccgc cccgaccagc 841 cggcggcctt cggcctcctg tgcagaatgg ccgaccagac cttcatctcc atcgtggact 901 gggcacgcag gtgcatggtc ttcaaggagc tggaggtggc cgaccagatg acgctgctgc 961 agaactgctg gagcgagctg ctggtgttcg accacatcta ccgccaggtc cagcacggca 1021 aggagggcag catcctgctg gtcaccgggc aggaggtgga gctgaccaca gtggccaccc 1081 aggcgggctc gctgctgcac agcctggtgt tgcgggcgca ggagctggtg ctgcagctgc 1141 ttgcgctgca gctggaccgg caggagtttg tctgccTCAA GTTCATCATC CTCTTCAGCC 1201 TGGATTTGAA GTTCCTGAAT AACCACATCC TGGTGAAAGA CGCTCAGGAG AAGGCCAACG 1261 CCGCCCTGCT TGACTACACC CTGTGCCACT ACCCGCACTG CGGGGACAAA TTCCAGCAGC 1321 TGCTGCTGTG CCTGGTGGAG GTGCGGGCCC TGAGCATGCA GGCCAAGGAG TACCTGTACC 1381 ACAAGCACCT GGGCAACGAG ATGCCCCGCA ACAACCTGCT CATCGAAATG CTGCAAGCCA 1441 AGCAGACTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1501 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1561 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACAATGT GATTCGAGGC CACTGCTTAC 1621 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt