Construct: ORF TRCN0000473374
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010972.1_s317c1
- Derived from:
- ccsbBroadEn_02899
- DNA Barcode:
- GTACACACCTCTCGCAGCGCTAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLIP3 (25999)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473374
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25999 | CLIP3 | CAP-Gly domain containing l... | NM_001199570.2 | 100% | 100% | |
| 2 | human | 25999 | CLIP3 | CAP-Gly domain containing l... | NM_015526.3 | 100% | 100% | |
| 3 | mouse | 76686 | Clip3 | CAP-GLY domain containing l... | NM_001081114.1 | 89.3% | 98.5% | (many diffs) |
| 4 | mouse | 76686 | Clip3 | CAP-GLY domain containing l... | XM_006540410.3 | 89.3% | 98.5% | (many diffs) |
| 5 | mouse | 76686 | Clip3 | CAP-GLY domain containing l... | XM_006540411.3 | 89.3% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1707
- ORF length:
- 1641
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac taagacagat cctgccccga tggccccgcc accccgagga gaggaggaag 121 aagaggagga ggaggatgaa cccgtccccg aggcccccag ccccacccag gagcgccggc 181 agaagcctgt tgtgcacccc tcggcacctg cccccctccc taaggactac gctttcacct 241 tcttcgatcc caatgacccg gcgtgccagg agatcctgtt tgaccctcag accaccatcc 301 ccgagctgtt tgccattgtg cgccagtggg tgccccaagt ccagcacaag atagacgtca 361 tcggcaatga gattctgcgc cgaggctgcc atgtgaacga tcgtgacggg ctgaccgaca 421 tgacactgct ccactatgcg tgcaaagctg gggcccacgg agtcggggac cccgcggcag 481 ccgtgcgcct ctcgcagcag ctgctggcgc tgggcgcaga tgtgacgctg cgcagccgct 541 ggaccaacat gaacgcgctt cactacgcgg cctattttga tgtgcccgac ctcgtgcgtg 601 tgctgctgaa gggtgcgagg ccgcgagtgg tgaactccac gtgcagtgac ttcaaccacg 661 gctcagccct gcacatcgct gcttccagcc tgtgcctggg cgccgccaaa tgtttgctgg 721 agcacggcgc caaccctgcg ctgaggaatc gaaaaggaca ggtgccggcg gaggtggtcc 781 cagatcctat ggacatgtcc ctggacaagg cagaggcggc actggtggcc aaggagctgc 841 ggacgcttct ggaagaggca gtgccactat cttgcgccct ccccaaggtc acgctaccca 901 actatgacaa cgtcccaggc aatctcatgc ttagcgcact gggcttgcgc ctgggagacc 961 gcgtgctgct ggatggccag aagacgggca cactgcggtt ctgtgggacc acggagtttg 1021 ccagcggcca gtgggtgggc gtggagctgg acgaacctga gggcaagaac gatggcagcg 1081 ttgggggcgt tcggtacttc atctgccctc ccaagcaggg tctctttgcc tccgtgtcca 1141 agatctccaa ggcagtggac gcacccccct cctctgtcac ctccacaccc cggacccccc 1201 ggatggACTT CTCCCGTGTC ACCGGCAAAG GCCGCAGGGA ACACAAAGGC AAGAAGAAGA 1261 CCCCATCATC CCCATCTCTG GGCAGCTTGC AGCAGCGTGA CGGGGCCAAG GCTGAGGTTG 1321 GAGACCAGGT CCTTGTCGCG GGCCAGAAGC AGGGGATCGT GCGCTTCTAC GGGAAGACAG 1381 ACTTTGCCCC AGGTTACTGG TATGGCATTG AGCTGGACCA GCCCACAGGC AAGCATGATG 1441 GCTCTGTCTT CGGTGTCCGG TACTTCACTT GCCCCCCGAG GCATGGGGTC TTCGCACCAG 1501 CATCCCGTAT TCAGAGGATT GGCGGATCCA CTGATTCCCC CGGGGACAGC GTTGGAGCCA 1561 AAAAAGTGCA TCAAGTGACA ATGACGCAGC CCAAACGCAC CTTCACCACA GTCCGGACCC 1621 CAAAGGACAT TGCATCAGAG AACTCCATTT CCAGGTTGCT GTTCTGCTGC TGGTTCCCCT 1681 GGATGCTGAG GGCGGAGATG CAGTCTTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1741 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1801 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTACACAC 1861 CTCTCGCAGC GCTAGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1921 aagatt