Construct: ORF TRCN0000473378
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012363.1_s317c1
- Derived from:
- ccsbBroadEn_14411
- DNA Barcode:
- GTAATTTCGTTCCCAATATCCCTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- C8orf37 (157657)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473378
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 157657 | C8orf37 | chromosome 8 open reading f... | NM_177965.4 | 99.8% | 36.2% | 217delG |
| 2 | human | 157657 | C8orf37 | chromosome 8 open reading f... | NM_001363260.1 | 84.3% | 42.8% | 217delG;372_373ins96 |
| 3 | mouse | 67157 | 2610301B20Rik | RIKEN cDNA 2610301B20 gene | NM_026005.3 | 84.6% | 26.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 315
- ORF length:
- 246
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggaggac ctggacgagc tcttggatga agtcgagtcc aagttttgca 121 cacctgacct tctaagacgg ggtatggtcg agcagcccaa aggctgcggc ggcggcaccc 181 acagtagcga ccggaaccaa gccaaggcga aagagacgct cagatcaaca gaaacattta 241 aaaaagaaga tgatcttgac agTCTTATTA ATGAAATACT TGAAAGCCCA ACTTGGACAA 301 AAAACCCTCT AAATTAAAAT CTAAATCTTC AGGTAACACA TCTGTCAGAG CTTCCATTGA 361 AGGCCTTGGT AAAAGTTGCA GTCCGGTGTA CCTTGGTGGA AGCTCTATTC CATGTGGGAT 421 TGGAACAAAT ATTTCATGGA GAGCATGTGA CCATCTGCGT TGTATAGCCT GTGATTTCTT 481 GGTAGTCAGC TATGATGACT ATATGTGGGA CAAATCGTGT GATTATCTGT TTTTCAGGAA 541 CAACATGCCA GAATTTCACA AATTAAAAGC AAAGTTGATA AAGAAGAAAG GAACACGGGC 601 ATATGCCTGC CAGTGTAGCT GGAGAACTAT TGAAGAAGTG ACTGACCTTC AGACAGATCA 661 TCAGCTTCGC TGGGTTTGTG GTAAACATTT GCCAACTTTC TTGTACAAAG TGGTTGATAT 721 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 781 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTAATT 841 TCGTTCCCAA TATCCCTAAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 901 gaaagatt