Construct: ORF TRCN0000473382
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003065.1_s317c1
- Derived from:
- ccsbBroadEn_15316
- DNA Barcode:
- TGCCATTTGCTTCAGTTCCGATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCTD8 (386617)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473382
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 386617 | KCTD8 | potassium channel tetrameri... | NM_198353.3 | 99.9% | 100% | 1269A>G |
| 2 | human | 386617 | KCTD8 | potassium channel tetrameri... | XM_011513690.3 | 94.3% | 94.4% | 962_1045del;1353A>G |
| 3 | mouse | 243043 | Kctd8 | potassium channel tetrameri... | NM_175519.5 | 88.4% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1488
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctctgaag gacacgggca gcggcggcag caccatcctg cccattagcg 121 agatggtttc ctcgtccagc tcgcccggcg cgtcggccgc cgccgccccg gggccctgcg 181 caccctcgcc cttccctgaa gtagtggagc tgaacgtagg cggccaggtt tatgtgacca 241 agcactcgac gctgctcagc gtcccggaca gtactttggc cagcatgttc tcgccctcta 301 gtccccgtgg cggcgcccgg cgccggggcg agctgcccag ggacagccgg gcgcgcttct 361 tcatcgaccg ggacggcttc cttttcaggt acgtgctgga ttatctgcgg gacaagcaac 421 tcgcgctgcc ggagcacttc cccgagaagg agcggctgct gcgcgaggcc gagtatttcc 481 agctcaccga cttggtcaag ctgctgtcgc ccaaggtcac caagcagaac tctctcaacg 541 acgagggctg ccagagcgac ctggaggaca acgtctcgca gggtagcagc gacgcgctgc 601 tgctgcgcgg ggcggcggcc gccgtgccct cgggcccggg agcgcacggt ggtggcggcg 661 gcggcggcgc gcaggacaag cgctcgggct tcctcacgct gggctaccgg ggctcctaca 721 ccaccgtgcg cgacaaccag gccgacgcca aattccggcg tgtggcgcgc atcatggtgt 781 gcgggcgcat cgcgctggcc aaggaggtct tcggggacac gctcaacgag agccgcgacc 841 ccgaccggca gccggagaag tacacgtccc gcttctacct caagttcacc tacttggagc 901 aggcctttga tcgcctgtcc gaggccggct tccacatggt ggcgtgtaac tcctcgggca 961 ccgccgcctt cgtcaaccag taccgcgacg acaagatctg gagcagctac accgagtaca 1021 ttttcttccg accaccTCAG AAAATAGTAT CACCTAAACA AGAACATGAA GATAGGAAAC 1081 ATGACAAAGT CACTGATAAA GGAAGTGAAA GTGGGACTTC CTGTAATGAG CTCTCCACTT 1141 CCAGTTGTGA CAGCCATTCA GAGGCAAGCA CTCCCCAGGA CAACCCATCC AGTGCCCAGC 1201 AGGCAACAGC TCACCAACCT AACACTTTAA CATTGGATCG CCCCTCTAAA AAAGCACCTG 1261 TACAATGGAT ACCCCCACCA GACAAACGCA GAAACAGTGA ACTCTTTCAG ACCCTCATCA 1321 GCAAGTCCCG GGAAACGAAT CTGTCCAAAA AGAAAGTCTG TGAGAAGCTA AGTGTGGAAG 1381 AAGAAATGAA AAAGTGTATT CAGGATTTTA AAAAAATCCA CATTCCAGAT TATTTTCCAG 1441 AGCGCAAACG CCAATGGCAA TCTGAACTGT TGCAGAAGTA TGGGTTATTG CCAACTTTCT 1501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1621 GTGGAAAGGA CGATGCCATT TGCTTCAGTT CCGATTTACG CGTTAAGTCg acaatcaacc 1681 tctggattac aaaatttgtg aaagatt