Construct: ORF TRCN0000473384
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017834.1_s317c1
- Derived from:
- ccsbBroadEn_11387
- DNA Barcode:
- TGTCGGAGACAGTTAAATCCTGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APBA3 (9546)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473384
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9546 | APBA3 | amyloid beta precursor prot... | XM_006722951.3 | 81.4% | 59.9% | (many diffs) |
| 2 | human | 9546 | APBA3 | amyloid beta precursor prot... | NM_004886.4 | 57.7% | 57.7% | 1_726del;1005G>A;1126T>C |
| 3 | human | 9546 | APBA3 | amyloid beta precursor prot... | XM_006722950.4 | 51.1% | 39% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1065
- ORF length:
- 999
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccaggcccgg gaggccatgg accgcgtcaa ggcccccgat ggggagaccc 121 agcccatgac ggaggtggac ctgttcgtct ccaccaagag gatcaaggtc ttgacagcgg 181 actcccagga ggccatgatg gaccacgccc tgcataccat ctcctacaca gccgacatcg 241 gctgcgtgct ggtgctgatg gcgcggcggc ggctggcacg gaggccggca ccccaggacc 301 acggccgccg cctctacaag atgctctgcc acgtattcta cgcagaggac gcccagctca 361 tcgcccaggc cattggccag gccttcgccg ccgcctacag ccagttccta cgggaaagcg 421 gtattgaccc cagccaggtg ggcgtgcacc cgagcccagg cgcccgccac ctccataatg 481 gggacctgga ccacttctcc aacagtgaca actgccggga ggtgcacctc gagaagcggc 541 gaggggaggg cctgggcgtg gccctggtgg agtcgggctg gggctccctg ctgcccacag 601 ccgtcatcgc caacctgctg cacggggggC CTGCTGAGCG CTCGGGGGCC CTCAGCATCG 661 GGGACCGCCT GACCGCCATC AACGGGACCA GCCTGGTGGG GCTGCCCCTG GCTGCGTGCC 721 AGGCCGCTGT CCGCGAGACG AAGTCGCAGA CGTCGGTGAC ACTCAGCATC GTCCACTGCC 781 CTCCCGTCAC CACCGCCATC ATCCACCGGC CCCACGCCCG CGAGCAGCTG GGCTTCTGCG 841 TGGAGGACGG CATCATCTGC AGCCTCCTCC GTGGTGGCAT CGCCGAGCGT GGGGGCATCC 901 GCGTCGGCCA CCGCATCATT GAGATCAATG GGCAGAGTGT GGTGGCCACG CCACACGCCC 961 GCATCATCGA GCTGCTCACC GAGGCCTATG GCGAGGTGCA TATCAAGACG ATGCCAGCTG 1021 CCACATATCG CCTCCTTACA GGCCAGGAGC AGCCCGTGTA CCTGTGCCCA ACTTTCTTGT 1081 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1141 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1201 GAAAGGACGA TGTCGGAGAC AGTTAAATCC TGATACGCGT TAAGTCgaca atcaacctct 1261 ggattacaaa atttgtgaaa gatt